دورية أكاديمية
Comprehensive DNA copy number profile and BAC library construction of an Indian individual.
| العنوان: | Comprehensive DNA copy number profile and BAC library construction of an Indian individual. |
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| المؤلفون: | Chakrabarty S; Division of Biotechnology, Manipal Life Sciences Centre, Manipal University, Manipal 576104, Karnataka state, India. sanjiban.c@manipal.edu, D'Souza RR, Bellampalli R, Rotti H, Saadi AV, Gopinath PM, Acharya RV, Govindaraj P, Thangaraj K, Satyamoorthy K |
| المصدر: | Gene [Gene] 2012 Jun 01; Vol. 500 (2), pp. 186-93. Date of Electronic Publication: 2012 Mar 21. |
| نوع المنشور: | Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier/North-Holland Country of Publication: Netherlands NLM ID: 7706761 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-0038 (Electronic) Linking ISSN: 03781119 NLM ISO Abbreviation: Gene Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Amsterdam, Elsevier/North-Holland, 1976- |
| مواضيع طبية MeSH: | Chromosomes, Artificial, Bacterial* , Genomic Library*, Chromosome Mapping/*methods , Comparative Genomic Hybridization/*methods , DNA Copy Number Variations/*genetics , Genome, Human/*genetics, Cell Line, Transformed ; DNA Primers/genetics ; Electrophoresis, Gel, Pulsed-Field ; Genomics ; Humans ; In Situ Hybridization, Fluorescence ; India ; Male ; Polymorphism, Single Nucleotide ; Real-Time Polymerase Chain Reaction ; Reproducibility of Results ; Restriction Mapping ; Sequence Analysis, DNA |
| مستخلص: | Bacterial artificial chromosomes (BACs) are used in genomic variation studies due to their capacity to carry a large insert, their high clonal stability, low rate of chimerism and ease of manipulation. In the present study, an attempt was made to create the first genomic BAC library of an anonymous Indian male (IMBL4) consisting of 100,224 clones covering the human genome more than three times. Restriction mapping of 255 BAC clones by pulse field gel electrophoresis confirmed an average insert size of 120 kb. The library was screened by PCR using SHANK3 (SH3 and multiple ankyrin repeat domains 3) and OLFM3 (olfactomedin 3) specific primers. A selection of clones was analyzed by fluorescent in situ hybridization (FISH) and sequencing. Fine mapping of copy number variable regions by array based comparative genomic hybridization identified 467 CNVRs in the IMBL4 genome. The IMBL4 BAC library represents the first cataloged Indian genome resource for applications in basic and clinical research. (Copyright © 2012 Elsevier B.V. All rights reserved.) |
| المشرفين على المادة: | 0 (DNA Primers) |
| تواريخ الأحداث: | Date Created: 20120403 Date Completed: 20120710 Latest Revision: 20120507 |
| رمز التحديث: | 20240628 |
| DOI: | 10.1016/j.gene.2012.03.054 |
| PMID: | 22465536 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1879-0038 |
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| DOI: | 10.1016/j.gene.2012.03.054 |