دورية أكاديمية
أعرض في EDS

The transcriptome of Lutzomyia longipalpis (Diptera: Psychodidae) male reproductive organs.

التفاصيل البيبلوغرافية
العنوان: The transcriptome of Lutzomyia longipalpis (Diptera: Psychodidae) male reproductive organs.
المؤلفون: Azevedo RV; Instituto Oswaldo Cruz, FIOCRUZ, Rio de Janeiro, Rio de Janeiro, Brazil., Dias DB, Bretãs JA, Mazzoni CJ, Souza NA, Albano RM, Wagner G, Davila AM, Peixoto AA
المصدر: PloS one [PLoS One] 2012; Vol. 7 (4), pp. e34495. Date of Electronic Publication: 2012 Apr 05.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Public Library of Science Country of Publication: United States NLM ID: 101285081 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1932-6203 (Electronic) Linking ISSN: 19326203 NLM ISO Abbreviation: PLoS One Subsets: MEDLINE
أسماء مطبوعة: Original Publication: San Francisco, CA : Public Library of Science
مواضيع طبية MeSH: Insect Proteins/*genetics , Insect Vectors/*genetics , Psychodidae/*genetics , Testis/*physiology , Transcriptome/*genetics, Amino Acid Sequence ; Animals ; Biomarkers/metabolism ; Computational Biology ; Expressed Sequence Tags ; Female ; Male ; Molecular Sequence Data ; Oligonucleotide Array Sequence Analysis ; Phylogeny ; RNA, Messenger/genetics ; Real-Time Polymerase Chain Reaction ; Reverse Transcriptase Polymerase Chain Reaction ; Sequence Homology, Amino Acid
مستخلص: Background: It has been suggested that genes involved in the reproductive biology of insect disease vectors are potential targets for future alternative methods of control. Little is known about the molecular biology of reproduction in phlebotomine sand flies and there is no information available concerning genes that are expressed in male reproductive organs of Lutzomyia longipalpis, the main vector of American visceral leishmaniasis and a species complex.
Methods/principal Findings: We generated 2678 high quality ESTs ("Expressed Sequence Tags") of L. longipalpis male reproductive organs that were grouped in 1391 non-redundant sequences (1136 singlets and 255 clusters). BLAST analysis revealed that only 57% of these sequences share similarity with a L. longipalpis female EST database. Although no more than 36% of the non-redundant sequences showed similarity to protein sequences deposited in databases, more than half of them presented the best-match hits with mosquito genes. Gene ontology analysis identified subsets of genes involved in biological processes such as protein biosynthesis and DNA replication, which are probably associated with spermatogenesis. A number of non-redundant sequences were also identified as putative male reproductive gland proteins (mRGPs), also known as male accessory gland protein genes (Acps).
Conclusions: The transcriptome analysis of L. longipalpis male reproductive organs is one step further in the study of the molecular basis of the reproductive biology of this important species complex. It has allowed the identification of genes potentially involved in spermatogenesis as well as putative mRGPs sequences, which have been studied in many insect species because of their effects on female post-mating behavior and physiology and their potential role in sexual selection and speciation. These data open a number of new avenues for further research in the molecular and evolutionary reproductive biology of sand flies.
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معلومات مُعتمدة: United States Howard Hughes Medical Institute
المشرفين على المادة: 0 (Biomarkers)
0 (Insect Proteins)
0 (RNA, Messenger)
تواريخ الأحداث: Date Created: 20120413 Date Completed: 20120821 Latest Revision: 20211021
رمز التحديث: 20231215
مُعرف محوري في PubMed: PMC3320635
DOI: 10.1371/journal.pone.0034495
PMID: 22496818
قاعدة البيانات: MEDLINE
الوصف
تدمد:1932-6203
DOI:10.1371/journal.pone.0034495