دورية أكاديمية
Candidate biomarker verification: Critical examination of a serum protein pattern for human colorectal cancer.
| العنوان: | Candidate biomarker verification: Critical examination of a serum protein pattern for human colorectal cancer. |
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| المؤلفون: | Albrethsen J; Clinical Biochemistry Unit, Department of Diagnostics, Glostrup Hospital, Glostrup, Denmark. j_albrethsen@hotmail.com, Bøgebo R, Møller CH, Olsen JA, Raskov HH, Gammeltoft S |
| المصدر: | Proteomics. Clinical applications [Proteomics Clin Appl] 2012 Apr; Vol. 6 (3-4), pp. 182-9. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Wiley-VCH Country of Publication: Germany NLM ID: 101298608 Publication Model: Print Cited Medium: Internet ISSN: 1862-8354 (Electronic) Linking ISSN: 18628346 NLM ISO Abbreviation: Proteomics Clin Appl Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Weinheim : Wiley-VCH, c2007- |
| مواضيع طبية MeSH: | Colorectal Neoplasms/*blood , Proteome/*analysis , Proteomics/*methods, Adult ; Aged ; Aged, 80 and over ; Biomarkers, Tumor/blood ; Blood Proteins/analysis ; Case-Control Studies ; Colorectal Neoplasms/diagnosis ; Colorectal Neoplasms/pathology ; Female ; Humans ; Male ; Mass Spectrometry/methods ; Middle Aged ; Multivariate Analysis ; Principal Component Analysis ; Proteolysis ; Reproducibility of Results ; Research Design ; Sensitivity and Specificity ; Specimen Handling |
| مستخلص: | Purpose: We critically examine a candidate serum protein pattern for human colorectal cancer (CRC) with respect to reproducibility, sample handling, and disease specificity. Experimental Design: Serum samples from CRC patients, patients with benign colon tumors and healthy individuals, were obtained at two collection sites and analyzed by SELDI-TOF MS on 8 days, over a period of 5 weeks. The spectra were subjected to multivariate analysis. Tissues from normal colon and CRC were analyzed by SELDI-TOF MS. Selected mass peaks were identified. Results: Using an elaborate experimental design we developed a multivariate classifier that correctly classified CRC and control serum measured on an independent day. The classifier did not discriminate between samples from CRC patients and patients with benign colon tumors, and, secondly, did not correctly classify serum from an independent collection site. All discriminatory mass peaks were identified as high abundant plasma proteins. Tissue profiling provided support of increased proteolytic activity in CRC tissue. Conclusion and Clinical Relevance: Critical verification did not justify advancing the identified CRC serum protein pattern into clinical validation without improvement. We believe that proteomics biomarker research could benefit if the presented, or a similar, verification scheme was more commonly employed in explorative biomarker studies. (© 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.) |
| المشرفين على المادة: | 0 (Biomarkers, Tumor) 0 (Blood Proteins) 0 (Proteome) |
| تواريخ الأحداث: | Date Created: 20120426 Date Completed: 20120806 Latest Revision: 20151119 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1002/prca.201100095 |
| PMID: | 22532454 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1862-8354 |
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| DOI: | 10.1002/prca.201100095 |