دورية أكاديمية
Neutrophil dysfunction induced by hyperglycemia: modulation of myeloperoxidase activity.
| العنوان: | Neutrophil dysfunction induced by hyperglycemia: modulation of myeloperoxidase activity. |
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| المؤلفون: | de Souza Ferreira C; Departamento de Análises Clínicas e Toxicológicas, Faculdade de Ciências Farmacêuticas, UNIFAL-MG, Alfenas, Minas Gerais, Brazil., Araújo TH, Ângelo ML, Pennacchi PC, Okada SS, de Araújo Paula FB, Migliorini S, Rodrigues MR |
| المصدر: | Cell biochemistry and function [Cell Biochem Funct] 2012 Oct; Vol. 30 (7), pp. 604-10. Date of Electronic Publication: 2012 May 20. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Wiley-Blackwell Country of Publication: England NLM ID: 8305874 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1099-0844 (Electronic) Linking ISSN: 02636484 NLM ISO Abbreviation: Cell Biochem Funct Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Oxford, England : Wiley-Blackwell Original Publication: Guildford, Surrey : Butterworth Scientific Ltd., c1983- |
| مواضيع طبية MeSH: | Hyperglycemia/*physiopathology , Neutrophils/*enzymology , Peroxidase/*metabolism, Animals ; Candida albicans/pathogenicity ; Hyperglycemia/metabolism ; Hypochlorous Acid/metabolism ; Luminescent Measurements ; Male ; NADPH Oxidases/metabolism ; Neutrophils/physiology ; Phagocytosis ; Rats ; Rats, Wistar |
| مستخلص: | Our data suggest that impaired activity of myeloperoxidase (MPO) may play an important role in the dysfunction of neutrophils from hyperglycemic rats. Neutrophil biochemical pathways include the NADPH oxidase system and the MPO enzyme. They both play important role in the killing function of neutrophils. The effect of hyperglycemia on the activity of these enzymes and the consequences with regard to Candida albicans phagocytosis and the microbicidal property of rat peritoneal neutrophils is evaluated here. The NADPH oxidase system activity was measured using chemiluminescence and cytochrome C reduction assays. MPO activity was measured by monitoring HOCl production, and MPO protein expression was analysed using Western blot and immunofluorescence. C. albicans phagocytosis and death were evaluated by optical microscopy using the May-Grunwald-Giemsa staining method. ROS generation kinetic was slightly delayed in the diabetic group. MPO expression levels were higher in diabetic neutrophils; however, MPO activity was decreased in these same neutrophils compared with the controls. C. albicans phagocytosis and killing were lower in the diabetic neutrophils. Based on our experimental model, the phagocytic and killing functions of neutrophil phagocytosis are impaired in diabetic rats because of the decreased production of HOCl, highlighting the importance of MPO in the microbicidal function of neutrophils. (Copyright © 2012 John Wiley & Sons, Ltd.) |
| المشرفين على المادة: | 712K4CDC10 (Hypochlorous Acid) EC 1.11.1.7 (Peroxidase) EC 1.6.3.- (NADPH Oxidases) |
| تواريخ الأحداث: | Date Created: 20120522 Date Completed: 20130220 Latest Revision: 20171116 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1002/cbf.2840 |
| PMID: | 22610543 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1099-0844 |
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| DOI: | 10.1002/cbf.2840 |