دورية أكاديمية
A novel FADS1 isoform potentiates FADS2-mediated production of eicosanoid precursor fatty acids.
| العنوان: | A novel FADS1 isoform potentiates FADS2-mediated production of eicosanoid precursor fatty acids. |
|---|---|
| المؤلفون: | Park WJ; Division of Nutritional Sciences, Cornell University, Ithaca, NY., Kothapalli KS, Reardon HT, Lawrence P, Qian SB, Brenna JT |
| المصدر: | Journal of lipid research [J Lipid Res] 2012 Aug; Vol. 53 (8), pp. 1502-12. Date of Electronic Publication: 2012 May 22. |
| نوع المنشور: | Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Country of Publication: United States NLM ID: 0376606 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1539-7262 (Electronic) Linking ISSN: 00222275 NLM ISO Abbreviation: J Lipid Res Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2021- : [New York] : Elsevier Original Publication: Memphis, Lipid Research, inc. |
| مواضيع طبية MeSH: | Eicosanoids/*biosynthesis , Fatty Acid Desaturases/*genetics , Fatty Acid Desaturases/*metabolism, Alternative Splicing ; Animals ; Animals, Newborn ; Cell Differentiation ; Cell Line, Tumor ; Computational Biology ; Delta-5 Fatty Acid Desaturase ; Embryo, Mammalian/cytology ; Exons/genetics ; Gene Expression Regulation, Enzymologic ; Humans ; Intracellular Space/metabolism ; Isoenzymes/genetics ; Isoenzymes/metabolism ; Papio ; Poly A/genetics ; Poly A/metabolism ; Protein Transport ; RNA, Messenger/genetics ; RNA, Messenger/metabolism ; Transcription, Genetic |
| مستخلص: | The fatty acid desaturase (FADS) genes code for the rate-limiting enzymes required for the biosynthesis of long-chain polyunsaturated fatty acids (LCPUFA). Here we report discovery and function of a novel FADS1 splice variant. FADS1 alternative transcript 1 (FADS1AT1) enhances desaturation of FADS2, leading to increased production of eicosanoid precursors, the first case of an isoform modulating the enzymatic activity encoded by another gene. Multiple protein isoforms were detected in primate liver, thymus, and brain. In human neuronal cells, their expression patterns are modulated by differentiation and result in alteration of cellular fatty acids. FADS1, but not FADS1AT1, localizes to endoplasmic reticulum and mitochondria. Ribosomal footprinting demonstrates that all three FADS genes are translated at similar levels. The noncatalytic regulation of FADS2 desaturation by FADS1AT1 is a novel, plausible mechanism by which several phylogenetically conserved FADS isoforms may regulate LCPUFA biosynthesis in a manner specific to tissue, organelle, and developmental stage. |
| References: | Gene. 2005 Jan 3;344:1-20. (PMID: 15656968) Am J Clin Nutr. 2006 Jun;83(6 Suppl):1499S-1504S. (PMID: 16841860) Biochemistry. 2000 Oct 3;39(39):11948-54. (PMID: 11009608) Genome Res. 2007 Feb;17(2):145-55. (PMID: 17210929) Biochimie. 2009 Jun;91(6):791-5. (PMID: 19455748) Biochim Biophys Acta. 1990 Oct 1;1046(3):277-87. (PMID: 2223867) Development. 2010 Sep 1;137(17):2951-60. (PMID: 20667912) PLoS One. 2009 Oct 19;4(10):e7526. (PMID: 19838305) Gene. 2009 Oct 1;446(1):28-34. (PMID: 19573581) Nat Rev Neurosci. 2007 Nov;8(11):819-31. (PMID: 17895907) Semin Cell Dev Biol. 2005 Feb;16(1):39-47. (PMID: 15659338) Nat Genet. 2010 Feb;42(2):137-41. (PMID: 20037589) Mol Cell Biochem. 1995 Jan 26;142(2):163-70. (PMID: 7770068) Hum Mol Genet. 2010 Oct 1;19(19):3885-94. (PMID: 20639392) J Neurosci. 2009 Oct 14;29(41):12795-801. (PMID: 19828791) FEBS Lett. 1998 Jul 10;431(1):1-6. (PMID: 9684854) Genome Res. 2000 Jul;10(7):1001-10. (PMID: 10899149) BMC Genomics. 2008 Sep 16;9 Suppl 2:S3. (PMID: 18831794) Mol Biol Rep. 2010 Jun;37(5):2403-6. (PMID: 19693691) Biochim Biophys Acta. 2009 Jan;1792(1):14-26. (PMID: 18992329) Cold Spring Harb Perspect Biol. 2011 Jan 01;3(1):a000778. (PMID: 21084389) Nucleic Acids Res. 2009 Jul;37(Web Server issue):W273-6. (PMID: 19406924) Science. 2009 Apr 10;324(5924):218-23. (PMID: 19213877) Proc Natl Acad Sci U S A. 1993 Feb 1;90(3):1073-7. (PMID: 8430076) Nat Genet. 2010 Feb;42(2):105-16. (PMID: 20081858) Comput Chem. 2001 Dec;26(1):51-6. (PMID: 11765852) Biochem J. 1985 Jan 15;225(2):307-13. (PMID: 3977836) J Mol Endocrinol. 2006 Aug;37(1):147-61. (PMID: 16901931) Anal Biochem. 2002 Aug 15;307(2):348-60. (PMID: 12202254) FASEB J. 2007 Mar;21(3):647-55. (PMID: 17185752) J Lipid Res. 2009 Jun;50(6):1195-202. (PMID: 19202133) J Neurochem. 2003 Feb;84(4):840-53. (PMID: 12562527) Mol Reprod Dev. 2011 Apr;78(4):226-40. (PMID: 21509852) Prostaglandins Leukot Essent Fatty Acids. 2010 Apr-Jun;82(4-6):281-5. (PMID: 20236814) ACS Chem Biol. 2008 Oct 17;3(10):609-17. (PMID: 18817380) Nature. 2010 Jan 28;463(7280):457-63. (PMID: 20110989) J Lipid Res. 2010 Mar;51(3):472-9. (PMID: 19752397) Prostaglandins Leukot Essent Fatty Acids. 2003 Feb;68(2):73-95. (PMID: 12538072) Circulation. 2002 Nov 19;106(21):2747-57. (PMID: 12438303) Annu Rev Nutr. 2004;24:345-76. (PMID: 15189125) Endocrinology. 2005 Feb;146(2):751-9. (PMID: 15528307) J Biol Chem. 2002 Nov 8;277(45):42899-911. (PMID: 12196547) J Muscle Res Cell Motil. 2006;27(8):559-75. (PMID: 16819597) Biochim Biophys Acta. 1986 Apr 15;876(2):300-8. (PMID: 2869785) Genome Res. 2004 Jan;14(1):62-6. (PMID: 14707170) Cell. 2004 Jan 23;116(2):281-97. (PMID: 14744438) Gene. 2001 Mar 7;265(1-2):11-23. (PMID: 11255003) Cold Spring Harb Perspect Biol. 2010 Sep;2(9):a004887. (PMID: 20484388) Genomics. 2000 Jun 1;66(2):175-83. (PMID: 10860662) Curr Opin Lipidol. 2008 Jun;19(3):248-56. (PMID: 18460915) Nat Rev Mol Cell Biol. 2009 Nov;10(11):741-54. (PMID: 19773805) J Biol Chem. 1999 Dec 24;274(52):37335-9. (PMID: 10601301) Genome Res. 2007 Jun;17(6):669-81. (PMID: 17567988) Nucleic Acids Res. 2001 Jul 1;29(13):2850-9. (PMID: 11433032) Eur J Biochem. 1996 Nov 1;241(3):779-86. (PMID: 8944766) Trends Genet. 2006 Mar;22(3):119-22. (PMID: 16430990) J Biol Chem. 1991 Oct 25;266(30):19995-20000. (PMID: 1834642) |
| معلومات مُعتمدة: | T32 HD052471 United States HD NICHD NIH HHS; T32-HD-052471 United States HD NICHD NIH HHS |
| المشرفين على المادة: | 0 (Delta-5 Fatty Acid Desaturase) 0 (Eicosanoids) 0 (Isoenzymes) 0 (RNA, Messenger) 24937-83-5 (Poly A) EC 1.14.19.- (Fatty Acid Desaturases) EC 1.14.19.3 (FADS1 protein, human) EC 1.14.19.3 (FADS2 protein, human) |
| تواريخ الأحداث: | Date Created: 20120524 Date Completed: 20121123 Latest Revision: 20211203 |
| رمز التحديث: | 20240628 |
| مُعرف محوري في PubMed: | PMC3540860 |
| DOI: | 10.1194/jlr.M025312 |
| PMID: | 22619218 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1539-7262 |
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| DOI: | 10.1194/jlr.M025312 |