دورية أكاديمية
Antibacterial activity and cytotoxicity analysis of halistanol trisulphate from marine sponge Petromica citrina.
| العنوان: | Antibacterial activity and cytotoxicity analysis of halistanol trisulphate from marine sponge Petromica citrina. |
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| المؤلفون: | Marinho PR; Instituto de Microbiologia Paulo de Góes, Universidade Federal do Rio de Janeiro, Av. Carlos Chagas Filho, 373, Cidade Universitária, 21941-590, Rio de Janeiro, Brazil., Simas NK, Kuster RM, Duarte RS, Fracalanzza SE, Ferreira DF, Romanos MT, Muricy G, Giambiagi-Demarval M, Laport MS |
| المصدر: | The Journal of antimicrobial chemotherapy [J Antimicrob Chemother] 2012 Oct; Vol. 67 (10), pp. 2396-400. Date of Electronic Publication: 2012 Jun 22. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Oxford University Press Country of Publication: England NLM ID: 7513617 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1460-2091 (Electronic) Linking ISSN: 03057453 NLM ISO Abbreviation: J Antimicrob Chemother Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 1997- : London : Oxford University Press Original Publication: London, New York, Academic Press. |
| مواضيع طبية MeSH: | Anti-Bacterial Agents/*isolation & purification , Anti-Bacterial Agents/*pharmacology , Bacteria/*drug effects , Porifera/*chemistry , Sterols/*isolation & purification , Sterols/*pharmacology, Animals ; Anti-Bacterial Agents/chemistry ; Bacteria/cytology ; Cell Extracts/chemistry ; Cell Extracts/isolation & purification ; Cell Extracts/pharmacology ; Cell Line, Tumor/drug effects ; Cell Survival/drug effects ; Chemical Fractionation ; Magnetic Resonance Spectroscopy ; Mice ; Microbial Sensitivity Tests ; Microscopy |
| مستخلص: | Objectives: An aqueous extract and fraction from the marine sponge Petromica citrina have antibacterial activity. We performed a chemical and biological characterization of the antibiotic substance from P. citrina and investigated its mode of action on Staphylococcus aureus cells. Methods: The inhibitory activity of the aqueous extract of P. citrina was determined against 14 bacteria belonging to type strains and clinical antibiotic-resistant strains. The aqueous extract was fractionated under bioassay guidance and the bioactive substance was identified by its (1)H-NMR, (13)C-NMR and mass spectra. The MIC and the MBC of this substance were determined. This substance was also subjected to cytotoxic bioassays. The mode of action on S. aureus cells was investigated by light and transmission electron microscopy analysis. Results: P. citrina showed a large spectrum of activity against type strains and resistant-bacteria such as S. aureus, Staphylococcus epidermidis, Enterococcus faecalis, Mycobacterium fortuitum and Neisseria gonorrhoeae. The aqueous extract was fractionated and halistanol trisulphate (24ε,25-dimethylcholestane-2β,3α,6α-triol trisodium sulphate) was isolated for the first time from P. citrina. Halistanol trisulphate had a bactericidal effect on exponentially growing S. aureus cells at the MIC (512 mg/L). Cytotoxicity biossays showed moderate toxicity against cancer cell line L929 (fibrosarcoma). This substance apparently acts by damaging the cell membrane, with subsequent cell lysis. Conclusions: Halistanol trisulphate is a broad-spectrum antibiotic isolated from P. citrina with a mode of action involving disruption of the cytoplasmic membrane. It is a new candidate for research on antibacterial substances. |
| المشرفين على المادة: | 0 (Anti-Bacterial Agents) 0 (Cell Extracts) 0 (Sterols) 103065-20-9 (halistanol trisulfate) |
| تواريخ الأحداث: | Date Created: 20120626 Date Completed: 20130109 Latest Revision: 20120918 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1093/jac/dks229 |
| PMID: | 22729926 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1460-2091 |
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| DOI: | 10.1093/jac/dks229 |