دورية أكاديمية
Phylogenetic study of sponge associated bacteria from the Lakshadweep archipelago and the antimicrobial activities of their secondary metabolites.
| العنوان: | Phylogenetic study of sponge associated bacteria from the Lakshadweep archipelago and the antimicrobial activities of their secondary metabolites. |
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| المؤلفون: | Gopi M; CMLRE Field Research Station, Agatti Island, Lakshadweep, 682553, India. gpsakthi@gmail.com, Ajith Kumar TT, Balagurunathan R, Vinoth R, Dhaneesh KV, Rajasekaran R, Balasubramanian T |
| المصدر: | World journal of microbiology & biotechnology [World J Microbiol Biotechnol] 2012 Feb; Vol. 28 (2), pp. 761-6. Date of Electronic Publication: 2011 Aug 21. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Springer Country of Publication: Germany NLM ID: 9012472 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1573-0972 (Electronic) Linking ISSN: 09593993 NLM ISO Abbreviation: World J Microbiol Biotechnol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2005- : Berlin : Springer Original Publication: Oxford, OX, UK : Published by Rapid Communications of Oxford Ltd in association with UNESCO and in collaboration with the International Union of Microbiological Societies, c1990- |
| مواضيع طبية MeSH: | Bacteria/*metabolism , Fishes/*microbiology , Porifera/*microbiology, Acinetobacter/classification ; Acinetobacter/genetics ; Acinetobacter/metabolism ; Animals ; Bacteria/classification ; Bacteria/genetics ; Phylogeny ; RNA, Ribosomal, 16S/genetics |
| مستخلص: | Marine ecosystem of the Lakshadweep archipelago is unique and known to have a very high degree of biodiversity with a number of endemic flora and fauna. The present study focuses to isolate the endosymbiotic microorganism from sponges and its effectiveness against marine ornamental fish pathogens. The sponges were collected from Agatti island of Lakshadweep archipelago and identified as Clathria procera, Sigmadocia fibulata and Dysidea granulosa. In which, 15 different types of bacteria were isolated and screened against marine ornamental fish pathogens (A. hydrophila, Vibrio alginolyticus, V. harveyii, V. parahaemolyticus and Pseudomonas fluorescens). The strain S25 was found as potential bacteria based on their antimicrobial activity against the fish pathogens. Molecular identification of the potential strain (S25) of the 16S rRNA gene showed 99% identity with Acinetobacter sp. The sequenced 16 s rRNA gene with 1,081 bp in length was submitted in NCBI Genbank and Accession was obtained (GenBank Accession number HM004071). The strain exhibited high similarity (99%) with the 16S rRNA gene of Acinetobacter calcoaceticus from GenBank database. Crude extract obtained with acetone and ethyl acetate from extracellular products of S25 showed significant antimicrobial activity by disc diffusion assay using 1,500 μg/ml of crude extract. Extracellular metobolite of A. calcoaceticus was extracted by shake flask method and the crude extract was partially purified by thin layer chromatography. Partially purified crude extract showed significant inhibition zone of antimicrobial activity (A. hydrophila, V. alginolyticus, V. parahaemolyticus) and less similar activity against V. harveyii and P. fluorescens. This is the first report on A. calcoaceticus isolated from sponges of Lakshadweep archipelago and the studies are underway to characterize and purify the antimicrobial compounds of the potential bacteria. |
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| المشرفين على المادة: | 0 (RNA, Ribosomal, 16S) |
| تواريخ الأحداث: | Date Created: 20120719 Date Completed: 20130211 Latest Revision: 20211021 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1007/s11274-011-0860-x |
| PMID: | 22806873 |
| قاعدة البيانات: | MEDLINE |
Find this article in full text from Springer Verlag. 
Full Text Finder | تدمد: | 1573-0972 |
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| DOI: | 10.1007/s11274-011-0860-x |