دورية أكاديمية
Staining characteristics of Lonchocarpus cyanescens leaf extract on the testis of Sprague-Dawley rats.
| العنوان: | Staining characteristics of Lonchocarpus cyanescens leaf extract on the testis of Sprague-Dawley rats. |
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| المؤلفون: | Bassey RB; Department of Anatomy, Faculty of Basic Medical Sciences, University of Uyo, Nigeria. rosemary_bassey@yahoo.com, Bakare AA, Edagha IA, Osinubi AA, Oremosu AA |
| المصدر: | Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada [Microsc Microanal] 2012 Aug; Vol. 18 (4), pp. 840-3. Date of Electronic Publication: 2012 Jul 26. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Oxford University Press Country of Publication: England NLM ID: 9712707 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1435-8115 (Electronic) Linking ISSN: 14319276 NLM ISO Abbreviation: Microsc Microanal Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2023- : [Oxford] : Oxford University Press Original Publication: New York, N.Y. : Springer-Verlag New York, 1997- |
| مواضيع طبية MeSH: | Coloring Agents/*chemistry , Fabaceae/*chemistry , Plant Extracts/*chemistry , Plant Leaves/*chemistry , Testis/*chemistry, Animals ; Coloring Agents/isolation & purification ; Male ; Plant Extracts/isolation & purification ; Rats ; Rats, Sprague-Dawley ; Staining and Labeling |
| مستخلص: | The use of nonallergic, nontoxic, and eco-friendly natural dyes has become a matter of significant importance due to increased environmental awareness on the need to avoid hazardous synthetic dyes. This study was to determine the staining properties of the dye extract of Lonchocarpus cyanescens on histomorphology of the testis. Freshly cut leaves of L. cyanescens obtained from Akpan Ifia Inan village in Ikono local government area of Akwa Ibom state (latitude 5° 10' 12″ N; longitude 7° 48' 0″ E) were put into a plastic jar and boiling water was poured to cover the leaves. It was covered and left for an hour. The liquid was strained and potassium hydroxide was added to the dye water mixture to reach a pH of 9. A whisk was used to mix air into the liquid, and the mixuture was then allowed to sit until the blue indigo had settled to the bottom of the container. The dye was diluted with 70% ethanol to a concentration of 0.1 g/mL and was used to stain sections of testes. Its potential for use as a counterstain was also investigated. The testes sections were stained in shades of blue. The dye overshadowed the colors of haematoxylin and eosin. Preliminary phytochemical screening of L. cyanescens revealed that it contains alkaloids, saponins, flavonoids, and tannins. |
| المشرفين على المادة: | 0 (Coloring Agents) 0 (Plant Extracts) |
| تواريخ الأحداث: | Date Created: 20120727 Date Completed: 20130131 Latest Revision: 20120801 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1017/S1431927612000505 |
| PMID: | 22832176 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1435-8115 |
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| DOI: | 10.1017/S1431927612000505 |