دورية أكاديمية
أعرض في EDS

Propylthiouracil increases sodium/iodide symporter gene expression and iodide uptake in rat thyroid cells in the absence of TSH.

التفاصيل البيبلوغرافية
العنوان: Propylthiouracil increases sodium/iodide symporter gene expression and iodide uptake in rat thyroid cells in the absence of TSH.
المؤلفون: Sue M; Laboratory of Molecular Diagnostics, Department of Mycobacteriology, National Institute of Infectious Diseases, Tokyo, Japan., Akama T, Kawashima A, Nakamura H, Hara T, Tanigawa K, Wu H, Yoshihara A, Ishido Y, Hiroi N, Yoshino G, Kohn LD, Ishii N, Suzuki K
المصدر: Thyroid : official journal of the American Thyroid Association [Thyroid] 2012 Aug; Vol. 22 (8), pp. 844-52.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Mary Ann Liebert Publishers Country of Publication: United States NLM ID: 9104317 Publication Model: Print Cited Medium: Internet ISSN: 1557-9077 (Electronic) Linking ISSN: 10507256 NLM ISO Abbreviation: Thyroid Subsets: MEDLINE
أسماء مطبوعة: Original Publication: New York, NY : Mary Ann Liebert Publishers,
مواضيع طبية MeSH: Propylthiouracil/*pharmacology , Symporters/*biosynthesis , Thyroid Gland/*metabolism , Thyrotropin/*pharmacology, Animals ; Graves Disease/metabolism ; Iodides/metabolism ; Methimazole/pharmacology ; RNA, Messenger/metabolism ; Rats ; Real-Time Polymerase Chain Reaction ; Symporters/genetics ; Thyroid Gland/drug effects
مستخلص: Background: Propylthiouracil (PTU) and methimazole (MMI) are drugs that are widely used to treat Graves' disease. Although both exert an antithyroid effect primarily by blocking thyroid peroxidase activity, their molecular structure and other actions are different. We hypothesized that PTU and MMI may have differential effects on thyroid-specific gene expression and function.
Methods: The effects of PTU and MMI on thyroid-specific gene expression and function were examined in rat thyroid FRTL-5 cells using DNA microarray, reverse transcriptase (RT)-polymerase chain reaction (PCR), real-time PCR, Western blot, immunohistochemistry, and radioiodine uptake studies.
Results: DNA microarray analysis showed a marked increase in sodium/iodide symporter (NIS) gene expression after PTU treatment, whereas MMI had no effect. RT-PCR and real-time PCR analysis revealed that PTU-induced NIS mRNA levels were comparable to those elicited by thyroid-stimulating hormone (TSH). PTU increased 5'-1880-bp and 5'-1052-bp activity of the rat NIS promoter. While PTU treatment also increased NIS protein levels, the size of the induced protein was smaller than that induced by TSH, and the protein localized predominantly in the cytoplasm rather than the plasma membrane. Accumulation of (125)I in FRTL-5 cells was increased by PTU stimulation, but this effect was weaker than that produced by TSH.
Conclusions: We found that PTU induces NIS expression and iodide uptake in rat thyroid FRTL-5 cells in the absence of TSH. Although PTU and MMI share similar antithyroid activity, their effects on other thyroid functions appear to be quite different, which could affect their therapeutic effectiveness.
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المشرفين على المادة: 0 (Iodides)
0 (RNA, Messenger)
0 (Symporters)
4XE5NDT4K1 (sodium-iodide symporter)
554Z48XN5E (Methimazole)
721M9407IY (Propylthiouracil)
9002-71-5 (Thyrotropin)
تواريخ الأحداث: Date Created: 20120803 Date Completed: 20121226 Latest Revision: 20211021
رمز التحديث: 20240628
مُعرف محوري في PubMed: PMC3407387
DOI: 10.1089/thy.2011.0290
PMID: 22853729
قاعدة البيانات: MEDLINE
الوصف
تدمد:1557-9077
DOI:10.1089/thy.2011.0290