دورية أكاديمية
أعرض في EDS

Endothelial cell senescence is associated with disrupted cell-cell junctions and increased monolayer permeability.

التفاصيل البيبلوغرافية
العنوان: Endothelial cell senescence is associated with disrupted cell-cell junctions and increased monolayer permeability.
المؤلفون: Krouwer VJ; Department of Biomolecular Imaging, Faculty of Science, Institute of Biomembranes, Utrecht University, Padualaan 8, 3584 CH, Utrecht, The Netherlands. j.a.post@uu.nl., Hekking LH, Langelaar-Makkinje M, Regan-Klapisz E, Post JA
المصدر: Vascular cell [Vasc Cell] 2012 Aug 28; Vol. 4 (1), pp. 12. Date of Electronic Publication: 2012 Aug 28.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Publiverse Online S.R.L Country of Publication: England NLM ID: 101553155 Publication Model: Electronic Cited Medium: Internet ISSN: 2045-824X (Electronic) Linking ISSN: 2045824X NLM ISO Abbreviation: Vasc Cell Subsets: PubMed not MEDLINE
أسماء مطبوعة: Publication: Bucuresti, Romania : Publiverse Online S.R.L.
Original Publication: London : Biomed Central
مستخلص: Background: Cellular senescence is associated with cellular dysfunction and has been shown to occur in vivo in age-related cardiovascular diseases such as atherosclerosis. Atherogenesis is accompanied by intimal accumulation of LDL and increased extravasation of monocytes towards accumulated and oxidized LDL, suggesting an affected barrier function of vascular endothelial cells. Our objective was to study the effect of cellular senescence on the barrier function of non-senescent endothelial cells.
Methods: Human umbilical vein endothelial cells were cultured until senescence. Senescent cells were compared with non-senescent cells and with co-cultures of non-senescent and senescent cells. Adherens junctions and tight junctions were studied. To assess the barrier function of various monolayers, assays to measure permeability for Lucifer Yellow (LY) and horseradish peroxidase (PO) were performed.
Results: The barrier function of monolayers comprising of senescent cells was compromised and coincided with a change in the distribution of junction proteins and a down-regulation of occludin and claudin-5 expression. Furthermore, a decreased expression of occludin and claudin-5 was observed in co-cultures of non-senescent and senescent cells, not only between senescent cells but also along the entire periphery of non-senescent cells lining a senescent cell.
Conclusions: Our findings show that the presence of senescent endothelial cells in a non-senescent monolayer disrupts tight junction morphology of surrounding young cells and increases the permeability of the monolayer for LY and PO.
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تواريخ الأحداث: Date Created: 20120830 Date Completed: 20121224 Latest Revision: 20211021
رمز التحديث: 20231215
مُعرف محوري في PubMed: PMC3527188
DOI: 10.1186/2045-824X-4-12
PMID: 22929066
قاعدة البيانات: MEDLINE
الوصف
تدمد:2045-824X
DOI:10.1186/2045-824X-4-12