دورية أكاديمية
أعرض في EDS

In vitro and in vivo evaluation of microporous chitosan hydrogel/nanofibrin composite bandage for skin tissue regeneration.

التفاصيل البيبلوغرافية
العنوان: In vitro and in vivo evaluation of microporous chitosan hydrogel/nanofibrin composite bandage for skin tissue regeneration.
المؤلفون: Sudheesh Kumar PT; Amrita Centre for Nanosciences and Molecular Medicine, Amrita Institute of Medical Sciences and Research Centre, Amrita Vishwa Vidyapeetham University, Kochi, India., Raj NM, Praveen G, Chennazhi KP, Nair SV, Jayakumar R
المصدر: Tissue engineering. Part A [Tissue Eng Part A] 2013 Feb; Vol. 19 (3-4), pp. 380-92. Date of Electronic Publication: 2012 Oct 10.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Mary Ann Liebert, Inc Country of Publication: United States NLM ID: 101466659 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1937-335X (Electronic) Linking ISSN: 19373341 NLM ISO Abbreviation: Tissue Eng Part A Subsets: MEDLINE
أسماء مطبوعة: Original Publication: New Rochelle, NY : Mary Ann Liebert, Inc.
مواضيع طبية MeSH: Chitosan/*chemistry , Fibrin/*pharmacology , Nanoparticles/*administration & dosage , Regeneration/*physiology , Skin/*growth & development , Skin/*injuries , Wounds, Penetrating/*therapy, Animals ; Bandages ; Chitosan/pharmacology ; Equipment Design ; Equipment Failure Analysis ; Fibrin/chemistry ; Hydrogels/chemistry ; Hydrogels/pharmacology ; Male ; Materials Testing ; Miniaturization ; Nanoparticles/chemistry ; Porosity ; Rats ; Rats, Sprague-Dawley ; Regeneration/drug effects ; Skin/drug effects ; Skin, Artificial ; Treatment Outcome ; Wound Healing/drug effects ; Wounds, Penetrating/diagnosis
مستخلص: In this work, we have developed chitosan hydrogel/nanofibrin composite bandages (CFBs) and characterized using Fourier transform-infrared spectroscopy and scanning electron microscopy. The homogeneous distribution of nanofibrin in the prepared chitosan hydrogel matrix was confirmed by phosphotungstic acid-hematoxylin staining. The mechanical strength, swelling, biodegradation, porosity, whole-blood clotting, and platelet activation studies were carried out. In addition, the cell viability, cell attachment, and infiltration of the prepared CFBs were evaluated using human umbilical vein endothelial cells (HUVECs) and human dermal fibroblast (HDF) cells. It was found that the CFBs were microporous, flexible, biodegradable, and showed enhanced blood clotting and platelet activity compared to the one without nanofibrin. The prepared CFBs were capable of absorbing fluid and this was confirmed when immersed in phosphate buffered saline. Cell viability studies on HUVECs and HDF cells proved the nontoxic nature of the CFBs. Cell attachment and infiltration studies showed that the cells were found attached and proliferated on the CFBs. In vivo experiments were carried out in Sprague-Dawley rats and found that the wound healing occurred within 2 weeks when treated with CFBs than compared to the bare wound and wound treated with Kaltostat. The deposition of collagen was found to be more on CFB-treated wounds compared to the control. The above results proved the use of these CFBs as an ideal candidate for skin tissue regeneration and wound healing.
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المشرفين على المادة: 0 (Hydrogels)
9001-31-4 (Fibrin)
9012-76-4 (Chitosan)
تواريخ الأحداث: Date Created: 20120901 Date Completed: 20130711 Latest Revision: 20240322
رمز التحديث: 20240322
مُعرف محوري في PubMed: PMC3542877
DOI: 10.1089/ten.TEA.2012.0376
PMID: 22934717
قاعدة البيانات: MEDLINE
الوصف
تدمد:1937-335X
DOI:10.1089/ten.TEA.2012.0376