دورية أكاديمية
Formation of a PKCζ/β-catenin complex in endothelial cells promotes angiopoietin-1-induced collective directional migration and angiogenic sprouting.
| العنوان: | Formation of a PKCζ/β-catenin complex in endothelial cells promotes angiopoietin-1-induced collective directional migration and angiogenic sprouting. |
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| المؤلفون: | Oubaha M; Laboratory of Endothelial Cell Biology, Institut de recherches cliniques de Montréal, Université de Montréal, QC, Canada., Lin MI, Margaron Y, Filion D, Price EN, Zon LI, Côté JF, Gratton JP |
| المصدر: | Blood [Blood] 2012 Oct 18; Vol. 120 (16), pp. 3371-81. Date of Electronic Publication: 2012 Aug 30. |
| نوع المنشور: | Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Country of Publication: United States NLM ID: 7603509 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1528-0020 (Electronic) Linking ISSN: 00064971 NLM ISO Abbreviation: Blood Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2021- : [New York] : Elsevier Original Publication: New York, Grune & Stratton [etc.] |
| مواضيع طبية MeSH: | Angiopoietin-1/*pharmacology , Cell Movement/*physiology , Endothelium, Vascular/*metabolism , Neovascularization, Physiologic/*physiology , Protein Kinase C/*metabolism , beta Catenin/*metabolism, Adherens Junctions/metabolism ; Animals ; Animals, Genetically Modified ; Aorta/cytology ; Aorta/metabolism ; COS Cells ; Cattle ; Cell Movement/drug effects ; Cell Polarity ; Cells, Cultured ; Chlorocebus aethiops ; Embryo, Nonmammalian/cytology ; Embryo, Nonmammalian/metabolism ; Endothelium, Vascular/cytology ; Fluorescent Antibody Technique ; Intercellular Junctions/metabolism ; Microinjections ; Wound Healing ; Zebrafish/genetics ; Zebrafish/growth & development ; Zebrafish/metabolism |
| مستخلص: | Angiogenic sprouting requires that cell-cell contacts be maintained during migration of endothelial cells. Angiopoietin-1 (Ang-1) and vascular endothelial growth factor act oppositely on endothelial cell junctions. We found that Ang-1 promotes collective and directional migration and, in contrast to VEGF, induces the formation of a complex formed of atypical protein kinase C (PKC)-ζ and β-catenin at cell-cell junctions and at the leading edge of migrating endothelial cells. This complex brings Par3, Par6, and adherens junction proteins at the front of migrating cells to locally activate Rac1 in response to Ang-1. The colocalization of PKCζ and β-catenin at leading edge along with PKCζ-dependent stabilization of cell-cell contacts promotes directed and collective endothelial cell migration. Consistent with these results, down-regulation of PKCζ in endothelial cells alters Ang-1-induced sprouting in vitro and knockdown in developing zebrafish results in intersegmental vessel defects caused by a perturbed directionality of tip cells and by loss of cell contacts between tip and stalk cells. These results reveal that PKCζ and β-catenin function in a complex at adherens junctions and at the leading edge of migrating endothelial cells to modulate collective and directional migration during angiogenesis. |
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| معلومات مُعتمدة: | MOP-86464 Canada CAPMC CIHR; MOP-111031 Canada CAPMC CIHR; P01 HL032262 United States HL NHLBI NIH HHS; 5P01HL32262-29 United States HL NHLBI NIH HHS; R01 HL048801 United States HL NHLBI NIH HHS; 5R01HL048801-18 United States HL NHLBI NIH HHS |
| المشرفين على المادة: | 0 (Angiopoietin-1) 0 (beta Catenin) EC 2.7.11.1 (protein kinase C zeta) EC 2.7.11.13 (Protein Kinase C) |
| تواريخ الأحداث: | Date Created: 20120901 Date Completed: 20130117 Latest Revision: 20211021 |
| رمز التحديث: | 20231215 |
| مُعرف محوري في PubMed: | PMC3476545 |
| DOI: | 10.1182/blood-2012-03-419721 |
| PMID: | 22936663 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1528-0020 |
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| DOI: | 10.1182/blood-2012-03-419721 |