دورية أكاديمية
A model to measure fluid outflow in rabbit capsules post glaucoma implant surgery.
| العنوان: | A model to measure fluid outflow in rabbit capsules post glaucoma implant surgery. |
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| المؤلفون: | Nguyen DQ; Centre for Eye Research Australia, University of Melbourne, Royal Victorian Eye and Ear Hospital, Melbourne, Australia., Ross CM, Li YQ, Pandav S, Gardiner B, Smith D, How AC, Crowston JG, Coote MA |
| المصدر: | Investigative ophthalmology & visual science [Invest Ophthalmol Vis Sci] 2012 Oct 05; Vol. 53 (11), pp. 6914-9. Date of Electronic Publication: 2012 Oct 05. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Association For Research In Vision And Ophthalmology (Arvo) Country of Publication: United States NLM ID: 7703701 Publication Model: Electronic Cited Medium: Internet ISSN: 1552-5783 (Electronic) Linking ISSN: 01460404 NLM ISO Abbreviation: Invest Ophthalmol Vis Sci Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Brookline Ma : Association For Research In Vision And Ophthalmology (Arvo) Original Publication: St. Louis, Mosby. |
| مواضيع طبية MeSH: | Disease Models, Animal* , Molteno Implants*, Anterior Chamber/*surgery , Aqueous Humor/*physiology , Glaucoma/*metabolism, Animals ; Anterior Chamber/pathology ; Female ; Glaucoma/pathology ; Glaucoma/surgery ; Intraocular Pressure/physiology ; Prosthesis Implantation ; Rabbits ; Tonometry, Ocular |
| مستخلص: | Purpose: Prior models of glaucoma filtration surgery assess bleb morphology, which does not always reflect function. Our aim is to establish a model that directly measures tissue hydraulic conductivity of postsurgical outflow in rabbit bleb capsules following experimental glaucoma filtration surgery. Methods: Nine rabbits underwent insertion of a single-plate pediatric Molteno implant into the anterior chamber of their left eye. Right eyes were used as controls. The rabbits were then allocated to one of two groups. Group one had outflow measurements performed at 1 week after surgery (n = 5), and group two had measurements performed at 4 weeks (n = 4). Measurements were performed by cannulating the drainage tube ostium in situ with a needle attached to a pressure transducer and a fluid column at 15 mm Hg. The drop in the fluid column was measured every minute for 5 minutes. For the control eyes (n = 6), the anterior chamber of the unoperated fellow eye was cannulated. Animals were euthanized with the implant and its surrounding capsule dissected and fixed in 4% paraformaldehyde, and embedded in paraffin before 6-μm sections were cut for histologic staining. Results: By 7 days after surgery, tube outflow was 0.117 ± 0.036 μL/min/mm Hg at 15 mm Hg (mean ± SEM), whereas at 28 days, it was 0.009 ± 0.003 μL/min/mm Hg. Control eyes had an outflow of 0.136 ± 0.007 μL/min/mm Hg (P = 0.004, one-way ANOVA). Hematoxylin and eosin staining demonstrated a thinner and looser arrangement of collagenous tissue in the capsules at 1 week compared with that at 4 weeks, which had thicker and more densely arranged collagen. Conclusions: We describe a new model to directly measure hydraulic conductivity in a rabbit glaucoma surgery implant model. The principal physiologic endpoint of glaucoma surgery can be reliably quantified and consistently measured with this model. At 28 days post glaucoma filtration surgery, a rabbit bleb capsule has significantly reduced tissue hydraulic conductivity, in line with loss of implant outflow facility, and increased thickness and density of fibrous encapsulation. |
| تواريخ الأحداث: | Date Created: 20120908 Date Completed: 20130108 Latest Revision: 20130109 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1167/iovs.12-10438 |
| PMID: | 22956615 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1552-5783 |
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| DOI: | 10.1167/iovs.12-10438 |