دورية أكاديمية
أعرض في EDS

Specific Kv1.3 blockade modulates key cholesterol-metabolism-associated molecules in human macrophages exposed to ox-LDL.

التفاصيل البيبلوغرافية
العنوان: Specific Kv1.3 blockade modulates key cholesterol-metabolism-associated molecules in human macrophages exposed to ox-LDL.
المؤلفون: Yang Y; Department of Cardiology, Union Hospital, Huazhong University of Science and Technology, Wuhan, China., Wang YF, Yang XF, Wang ZH, Lian YT, Yang Y, Li XW, Gao X, Chen J, Shu YW, Cheng LX, Liao YH, Liu K
المصدر: Journal of lipid research [J Lipid Res] 2013 Jan; Vol. 54 (1), pp. 34-43. Date of Electronic Publication: 2012 Oct 24.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Elsevier Country of Publication: United States NLM ID: 0376606 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1539-7262 (Electronic) Linking ISSN: 00222275 NLM ISO Abbreviation: J Lipid Res Subsets: MEDLINE
أسماء مطبوعة: Publication: 2021- : [New York] : Elsevier
Original Publication: Memphis, Lipid Research, inc.
مواضيع طبية MeSH: Antibody Specificity*, Cholesterol/*metabolism , Kv1.3 Potassium Channel/*antagonists & inhibitors , Kv1.3 Potassium Channel/*immunology , Lipoproteins, LDL/*pharmacology , Macrophages/*drug effects , Macrophages/*metabolism, ATP Binding Cassette Transporter 1 ; ATP Binding Cassette Transporter, Subfamily G, Member 1 ; ATP-Binding Cassette Transporters/metabolism ; Acetyl-CoA C-Acetyltransferase/metabolism ; Apolipoprotein A-I/metabolism ; Biological Transport/drug effects ; CD36 Antigens/metabolism ; Cell Line ; Cell Membrane/drug effects ; Cell Membrane/metabolism ; Electrophysiological Phenomena/drug effects ; Gene Expression Regulation/drug effects ; Humans ; Kv1.3 Potassium Channel/metabolism ; Kv1.5 Potassium Channel/antagonists & inhibitors ; Kv1.5 Potassium Channel/immunology ; Kv1.5 Potassium Channel/metabolism ; Macrophages/cytology ; Monocytes/cytology ; Potassium/metabolism ; Scavenger Receptors, Class A/metabolism ; Scavenger Receptors, Class E/metabolism
مستخلص: Cholesterol-metabolism-associated molecules, including scavenger receptor class A (SR-A), lectin-like oxidized low-density lipoprotein receptor-1 (LOX-1), CD36, ACAT1, ABCA1, ABCG1, and scavenger receptor class B type I, can modulate cholesterol metabolism in the transformation from macrophages to foam cells. Voltage-gated potassium channel Kv1.3 has increasingly been demonstrated to play an important role in the modulation of macrophage function. Here, we investigate the role of Kv1.3 in modulating cholesterol-metabolism-associated molecules in human acute monocytic leukemia cell-derived macrophages (THP-1 macrophages) and human monocyte-derived macrophages exposed to oxidized LDL (ox-LDL). Human Kv1.3 and Kv1.5 channels (hKv1.3 and hKv1.5) are expressed in macrophages and form a heteromultimeric channel. The hKv1.3-E314 antibody that we had generated as a specific hKv1.3 blocker inhibited outward delayed rectifier potassium currents, whereas the hKv1.5-E313 antibody that we had generated as a specific hKv1.5 blocker failed. Accordingly, the hKv1.3-E314 antibody reduced percentage of cholesterol ester and enhanced apoA-I-mediated cholesterol efflux in THP-1 macrophages and human monocyte-derived macrophages exposed to ox-LDL. The hKv1.3-E314 antibody downregulated SR-A, LOX-1, and ACAT1 expression and upregulated ABCA1 expression in THP-1 macrophages and human monocyte-derived macrophages. Our results reveal that specific Kv1.3 blockade represents a novel strategy modulating cholesterol metabolism in macrophages, which benefits the treatment of atherosclerotic lesions.
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المشرفين على المادة: 0 (ABCA1 protein, human)
0 (ABCG1 protein, human)
0 (ATP Binding Cassette Transporter 1)
0 (ATP Binding Cassette Transporter, Subfamily G, Member 1)
0 (ATP-Binding Cassette Transporters)
0 (Apolipoprotein A-I)
0 (CD36 Antigens)
0 (Kv1.3 Potassium Channel)
0 (Kv1.5 Potassium Channel)
0 (Lipoproteins, LDL)
0 (OLR1 protein, human)
0 (Scavenger Receptors, Class A)
0 (Scavenger Receptors, Class E)
0 (oxidized low density lipoprotein)
97C5T2UQ7J (Cholesterol)
EC 2.3.1.9 (ACAT1 protein, human)
EC 2.3.1.9 (Acetyl-CoA C-Acetyltransferase)
RWP5GA015D (Potassium)
تواريخ الأحداث: Date Created: 20121027 Date Completed: 20130528 Latest Revision: 20211021
رمز التحديث: 20231215
مُعرف محوري في PubMed: PMC3520537
DOI: 10.1194/jlr.M023846
PMID: 23099443
قاعدة البيانات: MEDLINE
الوصف
تدمد:1539-7262
DOI:10.1194/jlr.M023846