دورية أكاديمية
In vivo detection and quantification of chemicals that enhance protein stability.
العنوان: | In vivo detection and quantification of chemicals that enhance protein stability. |
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المؤلفون: | Hailu TT; Howard Hughes Medical Institute, Department of Molecular, Cellular, and Developmental Biology, University of Michigan, Ann Arbor, MI 48109, USA., Foit L, Bardwell JC |
المصدر: | Analytical biochemistry [Anal Biochem] 2013 Mar 01; Vol. 434 (1), pp. 181-6. Date of Electronic Publication: 2012 Dec 05. |
نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
اللغة: | English |
بيانات الدورية: | Publisher: Elsevier Country of Publication: United States NLM ID: 0370535 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1096-0309 (Electronic) Linking ISSN: 00032697 NLM ISO Abbreviation: Anal Biochem Subsets: MEDLINE |
أسماء مطبوعة: | Publication: <2000- > : San Diego, CA : Elsevier Original Publication: Orlando Fl : Academic Press |
مواضيع طبية MeSH: | Colorimetry*, Carbohydrates/*analysis , Caseins/*analysis , Lipids/*analysis , Plant Proteins, Dietary/*analysis , beta-Lactamases/*metabolism, Escherichia coli/metabolism ; Molecular Chaperones/metabolism ; Protein Folding ; Protein Stability ; beta-Lactamases/chemistry |
مستخلص: | We have devised protein-folding sensors that link protein stability to TEM-1 β-lactamase activity. The addition of osmolytes and other compounds with chemical chaperone activity to the growth medium of bacteria containing these sensors increases β-lactamase activity up to 207-fold in a dose-dependent manner. This enables the rapid detection and sensitive quantification of compounds that enhance in vivo protein stability. (Copyright © 2012 Elsevier Inc. All rights reserved.) |
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معلومات مُعتمدة: | United States HHMI_ Howard Hughes Medical Institute |
فهرسة مساهمة: | Indexing Agency: NLM Local ID #: HHMIMS458709. |
المشرفين على المادة: | 0 (Carbohydrates) 0 (Caseins) 0 (Lipids) 0 (Molecular Chaperones) 0 (Plant Proteins, Dietary) 102257-18-1 (Osmolite) EC 3.5.2.6 (beta-Lactamases) EC 3.5.2.6 (beta-lactamase TEM-1) |
تواريخ الأحداث: | Date Created: 20121211 Date Completed: 20130814 Latest Revision: 20230425 |
رمز التحديث: | 20231215 |
مُعرف محوري في PubMed: | PMC3670414 |
DOI: | 10.1016/j.ab.2012.11.022 |
PMID: | 23219982 |
قاعدة البيانات: | MEDLINE |
تدمد: | 1096-0309 |
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DOI: | 10.1016/j.ab.2012.11.022 |