التفاصيل البيبلوغرافية
| العنوان: |
Spectrum of non-tuberculous mycobacteria identified using standard biochemical testing vs. 16S sequencing. |
| المؤلفون: |
Chihota VN; The Aurum Institute, Parktown, South Africa. vchihota@auruminstitute.org, van Halsema CL, Grant AD, Fielding KL, van Helden PD, Churchyard GJ, Gey van Pittius NC |
| المصدر: |
The international journal of tuberculosis and lung disease : the official journal of the International Union against Tuberculosis and Lung Disease [Int J Tuberc Lung Dis] 2013 Feb; Vol. 17 (2), pp. 267-9. Date of Electronic Publication: 2012 Dec 08. |
| نوع المنشور: |
Comparative Study; Journal Article; Multicenter Study; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't |
| اللغة: |
English |
| بيانات الدورية: |
Publisher: The Union Country of Publication: France NLM ID: 9706389 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1815-7920 (Electronic) Linking ISSN: 10273719 NLM ISO Abbreviation: Int J Tuberc Lung Dis Subsets: MEDLINE |
| أسماء مطبوعة: |
Original Publication: Paris, France : The Union, c1997- |
| مواضيع طبية MeSH: |
Bacterial Typing Techniques/*methods , DNA, Bacterial/*analysis , Mycobacterium/*classification , Tuberculosis/*microbiology, Humans ; Mycobacterium/genetics ; Mycobacterium/isolation & purification ; Polymerase Chain Reaction ; Reproducibility of Results ; Retrospective Studies ; Sequence Analysis, DNA ; Tuberculosis/diagnosis ; Tuberculosis/genetics |
| مستخلص: |
Non-tuberculous mycobacterial isolates from gold miners were speciated using standard biochemical testing (SBT) and 16S rDNA sequencing. Of 237 isolates tested, SBT identified 126, compared with all 237 identified using sequencing. Of 111 isolates unspeciated by SBT but identified by sequencing, 38 (34.2%) were identified as Mycobacterium gordonae and 8 (7.2%) were new species. Of 126 isolates speciated by both methods, 37 were discordant, with 14/17 M. gordonae isolates incorrectly identified as M. scrofulaceum using SBT. The majority of these were the potentially pathogenic strain D, M. gordonae. Sequencing is preferable where available to guide treatment. |
| معلومات مُعتمدة: |
MR/K012126/1 United Kingdom MRC_ Medical Research Council; 5U2RTW007373 United States PHS HHS; 5U2RTW007370 United States PHS HHS; United Kingdom DH_ Department of Health |
| المشرفين على المادة: |
0 (DNA, Bacterial) |
| تواريخ الأحداث: |
Date Created: 20121212 Date Completed: 20130702 Latest Revision: 20211021 |
| رمز التحديث: |
20221213 |
| DOI: |
10.5588/ijtld.12.0425 |
| PMID: |
23228479 |
| قاعدة البيانات: |
MEDLINE |
