دورية أكاديمية
Elastomeric negative acoustic contrast particles for affinity capture assays.
| العنوان: | Elastomeric negative acoustic contrast particles for affinity capture assays. |
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| المؤلفون: | Cushing KW; Department of Chemical and Nuclear Engineering, Center for Biomedical Engineering, The University of New Mexico, Albuquerque, New Mexico 87131, United States., Piyasena ME, Carroll NJ, Maestas GC, López BA, Edwards BS, Graves SW, López GP |
| المصدر: | Analytical chemistry [Anal Chem] 2013 Feb 19; Vol. 85 (4), pp. 2208-15. Date of Electronic Publication: 2013 Feb 05. |
| نوع المنشور: | Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S. |
| اللغة: | English |
| بيانات الدورية: | Publisher: American Chemical Society Country of Publication: United States NLM ID: 0370536 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1520-6882 (Electronic) Linking ISSN: 00032700 NLM ISO Abbreviation: Anal Chem Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Washington, American Chemical Society. |
| مواضيع طبية MeSH: | Microspheres*, Flow Cytometry/*methods , Polymers/*chemistry , Prostate-Specific Antigen/*analysis, Animals ; Antibodies, Monoclonal/immunology ; Biomarkers/analysis ; Biomarkers/blood ; Dimethylpolysiloxanes/chemistry ; Elastomers ; Humans ; Immunoglobulin G/blood ; Mice ; Microfluidic Analytical Techniques ; Polymers/chemical synthesis ; Swine |
| مستخلص: | This report describes the development of elastomeric capture microparticles (ECμPs) and their use with acoustophoretic separation to perform microparticle assays via flow cytometry.We have developed simple methods to form ECμPs by cross-linking droplets of common commercially available silicone precursors in suspension followed by surface functionalization with biomolecular recognition reagents. The ECμPs are compressible particles that exhibit negative acoustic contrast in ultrasound when suspended in aqueous media, blood serum, or diluted blood. In this study, these particles have been functionalized with antibodies to bind prostate specific antigen and immunoglobulin (IgG). Specific separation of the ECμPs from blood cells is achieved by flowing them through a microfluidic acoustophoretic device that uses an ultrasonic standing wave to align the blood cells, which exhibit positive acoustic contrast, at a node in the acoustic pressure distribution while aligning the negative acoustic contrast ECμPs at the antinodes. Laminar flow of the separated particles to downstream collection ports allows for collection of the separated negative contrast (ECμPs) and positive contrast particles (cells). Separated ECμPs were analyzed via flow cytometry to demonstrate nanomolar detection for prostate specific antigen in aqueous buffer and picomolar detection for IgG in plasma and diluted blood samples. This approach has potential applications in the development of rapid assays that detect the presence of low concentrations of biomarkers in a number of biological sample types. |
| References: | Lab Chip. 2011 Oct 7;11(19):3361-4. (PMID: 21842070) Bioconjug Chem. 2010 Dec 15;21(12):2197-204. (PMID: 21070026) Integr Biol (Camb). 2009 Jan;1(1):30-42. (PMID: 20023789) Curr Infect Dis Rep. 2006 Mar;8(2):125-31. (PMID: 16524549) J Immunol Methods. 2002 Feb 1;260(1-2):207-18. (PMID: 11792390) Ultrasonics. 2000 Mar;38(1-8):654-6. (PMID: 10829746) Lancet Infect Dis. 2002 Apr;2(4):243-50. (PMID: 11937424) Anal Chem. 2012 Feb 21;84(4):1831-9. (PMID: 22239072) Nat Nanotechnol. 2010 Feb;5(2):138-42. (PMID: 20010825) Lab Chip. 2012 Mar 21;12(6):1014-21. (PMID: 22349937) J Proteomics. 2011 Nov 18;74(12):2650-9. (PMID: 21540133) J Chromatogr B Analyt Technol Biomed Life Sci. 2004 Apr 15;803(1):27-40. (PMID: 15025996) Drug Discov Today. 1999 Apr;4(4):173-180. (PMID: 10322276) Adv Mater. 2011 Jun 24;23(24):H151-76. (PMID: 21567479) PLoS One. 2011;6(8):e23074. (PMID: 21857996) Anal Chem. 2009 Aug 1;81(15):6030-7. (PMID: 19594154) Methods. 2012 Jul;57(3):259-71. (PMID: 22465280) Anal Chem. 2009 Aug 1;81(15):6195-200. (PMID: 19572705) Biomed Microdevices. 2005 Dec;7(4):281-93. (PMID: 16404506) Clin Endocrinol (Oxf). 2003 Jun;58(6):686-90. (PMID: 12780743) Analyst. 2004 Oct;129(10):938-43. (PMID: 15457327) J Pharm Biomed Anal. 2012 Apr 7;63:165-9. (PMID: 22326845) PLoS Pathog. 2012;8(6):e1002739. (PMID: 22719248) Anal Bioanal Chem. 2010 Aug;397(8):3249-67. (PMID: 20419490) Chem Soc Rev. 2010 Mar;39(3):1203-17. (PMID: 20179832) Electrophoresis. 2004 May;25(9):1299-306. (PMID: 15174052) Nat Rev Cancer. 2003 Apr;3(4):243-52. (PMID: 12671663) Anal Bioanal Chem. 2010 Feb;396(3):1223-47. (PMID: 20033139) Annu Rev Biomed Eng. 2005;7:77-103. (PMID: 16004567) Int J Gynecol Cancer. 2003 Nov-Dec;13 Suppl 2:133-9. (PMID: 14656269) Cancer Res. 2005 May 15;65(10):4097-100. (PMID: 15899799) Lab Chip. 2005 Jan;5(1):20-2. (PMID: 15616735) Chem Soc Rev. 2007 Mar;36(3):492-506. (PMID: 17325788) |
| معلومات مُعتمدة: | R21 RR020064 United States RR NCRR NIH HHS; RR020064 United States RR NCRR NIH HHS; RR001315 United States RR NCRR NIH HHS; R25 GM060201 United States GM NIGMS NIH HHS; R33 RR020064 United States RR NCRR NIH HHS; P41 RR001315 United States RR NCRR NIH HHS |
| المشرفين على المادة: | 0 (Antibodies, Monoclonal) 0 (Biomarkers) 0 (Dimethylpolysiloxanes) 0 (Elastomers) 0 (Immunoglobulin G) 0 (Polymers) 0 (elastomeric polymer) EC 3.4.21.77 (Prostate-Specific Antigen) |
| تواريخ الأحداث: | Date Created: 20130122 Date Completed: 20131118 Latest Revision: 20240610 |
| رمز التحديث: | 20240610 |
| مُعرف محوري في PubMed: | PMC3621144 |
| DOI: | 10.1021/ac3029344 |
| PMID: | 23331264 |
| قاعدة البيانات: | MEDLINE |
Find this issue from the American Chemical Society | تدمد: | 1520-6882 |
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| DOI: | 10.1021/ac3029344 |