دورية أكاديمية
Deproteination of serum samples for LC-MS/MS analyses by applying magnetic micro-particles.
| العنوان: | Deproteination of serum samples for LC-MS/MS analyses by applying magnetic micro-particles. |
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| المؤلفون: | König K; Institute of Laboratory Medicine, Hospital of the University of Munich, Munich, Germany. katrin.koenig@med.uni-muenchen.de, Goethel SF, Rusu VM, Vogeser M |
| المصدر: | Clinical biochemistry [Clin Biochem] 2013 May; Vol. 46 (7-8), pp. 652-5. Date of Electronic Publication: 2013 Jan 18. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't; Validation Study |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Science Country of Publication: United States NLM ID: 0133660 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-2933 (Electronic) Linking ISSN: 00099120 NLM ISO Abbreviation: Clin Biochem Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Tarrytown, NY : Elsevier Science Original Publication: [Toronto] Canadian Society of Clinical Chemists. |
| مواضيع طبية MeSH: | Chromatography, High Pressure Liquid/*methods , Proteins/*isolation & purification , Serum/*chemistry , Tandem Mass Spectrometry/*methods, Amiodarone/blood ; Automation, Laboratory/methods ; Fractional Precipitation ; Humans ; Magnetics ; Protein Denaturation ; Reproducibility of Results |
| مستخلص: | Objectives: Investigation of the practicability and performance of a magnetic micro-particle based method for protein depletion of serum samples, preceding the quantitative analysis of small molecules by LC-MS/MS. Design and Methods: A commercially available kit including a protein denaturation reagent and functionalized magnetic particles together with a magnetic separator device was tested by addressing the quantification of amiodarone in serum as an exemplary analyte by LC-MS/MS with on-line SPE. A standard method validation protocol was applied. Results: The sample preparation protocol was found to be convenient, straightforward and robust. Validation data characterized the entire analytical method - combining particle-based protein depletion and two-dimensional chromatography - as compatible with the analytical needs regarding selectivity, accuracy (102-106%), linearity (r(2)≥0.99), reproducibility (CV<7%), and control of ion suppression. Conclusions: Since this novel approach of sample preparation does neither require centrifugation nor the technically demanding application of positive or negative pressure, as in conventional solid phase extraction protocols, it seems highly attractive for developing fully automatized preparation systems for LC-MS/MS analyzers. (Copyright © 2013 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved.) |
| المشرفين على المادة: | 0 (Proteins) N3RQ532IUT (Amiodarone) |
| تواريخ الأحداث: | Date Created: 20130123 Date Completed: 20130930 Latest Revision: 20191210 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1016/j.clinbiochem.2013.01.003 |
| PMID: | 23337691 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1873-2933 |
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| DOI: | 10.1016/j.clinbiochem.2013.01.003 |