دورية أكاديمية
Mouse renal 4T1 cell engraftment as a model to study the influence of hypoxia in breast cancer progression.
| العنوان: | Mouse renal 4T1 cell engraftment as a model to study the influence of hypoxia in breast cancer progression. |
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| المؤلفون: | Antonio HM; Breast Disease Unit, Department of Gynecology and Obstetrics, Ribeirao Preto School of Medicine, USP, Sao Paulo, Brazil., Mandarano LR, Coelho AA, Tiezzi MG, Andrade JM, Tiezzi DG |
| المصدر: | Acta cirurgica brasileira [Acta Cir Bras] 2013 Feb; Vol. 28 (2), pp. 142-7. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Sociedade Brasileira Para O Desenvolvimento Da Pesquisa Em Cirurgia Curso De Pos-Graduacao Em Tecnica Operatoria E Cirurgia Experimental Escola Paulista De Medicina Country of Publication: Brazil NLM ID: 9103983 Publication Model: Print Cited Medium: Internet ISSN: 1678-2674 (Electronic) Linking ISSN: 01028650 NLM ISO Abbreviation: Acta Cir Bras Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Sao Paulo : Sociedade Brasileira Para O Desenvolvimento Da Pesquisa Em Cirurgia Curso De Pos-Graduacao Em Tecnica Operatoria E Cirurgia Experimental Escola Paulista De Medicina Original Publication: São Paulo : A Sociedade, |
| مواضيع طبية MeSH: | Cell Hypoxia/*physiology , Kidney/*pathology , Lung Neoplasms/*secondary , Mammary Neoplasms, Experimental/*pathology, Animals ; Cell Line, Tumor ; Disease Progression ; Female ; Kidney/blood supply ; Mice ; Mice, Inbred BALB C ; Models, Animal ; Neoplasm Transplantation |
| مستخلص: | Purpose: To develop a mouse model to study the influence of hypoxia in breast cancer progression and metastasis. Methods: The 4T1 cell line was used to engraft the kidneys of female BALB/c mice. Placing an aneurysm clip on the kidney hilum, hypoxia can be directed to tumor site. Histological evaluation was used to analyze the morphological changes induced by ischemia in kidney cortex, and to verify the metastatic potential. Results: 4T1 cells can be engrafted into the renal cortex and the renal ischemia caused by using a clip to clamp the renal hilum induces hypoxia at the tumor site. This procedure maintains the ability of 4T1 cells to metastasize. In fact, our preliminary results showed that tumor hypoxia precipitates the metastatic dissemination of tumor cells. After 14 days of engraftment, lung metastases were observed only in mice that were subjected to tumor hypoxia. Conclusion: This model can help us to understand how low oxygen tension mediates hypoxia-induced proteomic and genomic changes in breast cancer. |
| تواريخ الأحداث: | Date Created: 20130202 Date Completed: 20131204 Latest Revision: 20191027 |
| رمز التحديث: | 20240628 |
| DOI: | 10.1590/s0102-86502013000200010 |
| PMID: | 23370929 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1678-2674 |
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| DOI: | 10.1590/s0102-86502013000200010 |