دورية أكاديمية
Bacteriocin PJ4 active against enteric pathogen produced by Lactobacillus helveticus PJ4 isolated from gut microflora of wistar rat (Rattus norvegicus): partial purification and characterization of bacteriocin.
| العنوان: | Bacteriocin PJ4 active against enteric pathogen produced by Lactobacillus helveticus PJ4 isolated from gut microflora of wistar rat (Rattus norvegicus): partial purification and characterization of bacteriocin. |
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| المؤلفون: | Jena PK; Institute of Science, Nirma University, Sarkhej-Gandhinagar Highway, Chharodi, Ahmedabad 382481 Gujarat, India., Trivedi D, Chaudhary H, Sahoo TK, Seshadri S |
| المصدر: | Applied biochemistry and biotechnology [Appl Biochem Biotechnol] 2013 Apr; Vol. 169 (7), pp. 2088-100. Date of Electronic Publication: 2013 Feb 01. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Humana Press Country of Publication: United States NLM ID: 8208561 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1559-0291 (Electronic) Linking ISSN: 02732289 NLM ISO Abbreviation: Appl Biochem Biotechnol Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Clifton, N.J. : Humana Press, c1981- |
| مواضيع طبية MeSH: | Anti-Bacterial Agents/*metabolism , Anti-Bacterial Agents/*pharmacology , Bacteriocins/*metabolism , Bacteriocins/*pharmacology , Lactobacillus helveticus/*metabolism, Animals ; Escherichia coli/drug effects ; Gram-Negative Bacteria/drug effects ; Gram-Positive Bacteria/drug effects ; Lactobacillus/drug effects ; Male ; Rats ; Rats, Wistar ; Staphylococcus aureus/drug effects |
| مستخلص: | The increase of multidrug-resistant pathogens and the restriction on the use antibiotics due to its side effects have drawn attention to the search for possible alternatives. Bacteriocins are small antimicrobial peptides produced by numerous bacteria. Much interest has been focused on bacteriocins because they exhibit inhibitory activity against pathogens. Lactic acid bacteria possess the ability to synthesize antimicrobial compounds (like bacteriocin) during their growth. In this study, an antibacterial substance (bacteriocin PJ4) produced by Lactobacillus helveticus PJ4, isolated from rat gut microflora, was identified as bacteriocin. It was effective against wide assay of both Gram-positive and Gram-negative bacteria involved in various diseases, including Escherichia coli, Bacillus subtilis, Pseudomonas aeruginosa, Enterococcus faecalis, and Staphylococcus aureus. The antimicrobial peptide was relatively heat-resistant and also active over a wide pH range of 2-10. It has been partially purified to homogeneity using ammonium sulfate precipitation and size exclusion chromatography and checked on reverse-phase high-performance liquid chromatography. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of bacteriocin PJ4 purified through size exclusion chromatography resolved ~6.5 kDa protein with bacteriocin activity. The peptide is inactivated by proteolytic enzymes, trypsin, and lipase but not when treated with catalase, α-amylase, and pepsin. It showed a bactericidal mode of action against the indicator strains E. coli MTCC443, Lactobacillus casei MTCC1423, and E. faecalis DT48. Such characteristics indicate that this bacteriocin may be a potential candidate for alternative agents to control important pathogens. |
| المشرفين على المادة: | 0 (Anti-Bacterial Agents) 0 (Bacteriocins) |
| تواريخ الأحداث: | Date Created: 20130202 Date Completed: 20130909 Latest Revision: 20130321 |
| رمز التحديث: | 20240628 |
| DOI: | 10.1007/s12010-012-0044-7 |
| PMID: | 23371780 |
| قاعدة البيانات: | MEDLINE |
Find this article in full text from Springer Verlag. 
Full Text Finder | تدمد: | 1559-0291 |
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| DOI: | 10.1007/s12010-012-0044-7 |