دورية أكاديمية
Detection of Coxiella burnetii DNA in the environment during and after a large Q fever epidemic in the Netherlands.
| العنوان: | Detection of Coxiella burnetii DNA in the environment during and after a large Q fever epidemic in the Netherlands. |
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| المؤلفون: | de Bruin A; National Institute for Public Health and the Environment, Bilthoven, The Netherlands. arnout.de.bruin@rivm.nl, Janse I, Koning M, de Heer L, van der Plaats RQ, van Leuken JP, van Rotterdam BJ |
| المصدر: | Journal of applied microbiology [J Appl Microbiol] 2013 May; Vol. 114 (5), pp. 1395-404. Date of Electronic Publication: 2013 Feb 27. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Oxford University Press Country of Publication: England NLM ID: 9706280 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1365-2672 (Electronic) Linking ISSN: 13645072 NLM ISO Abbreviation: J Appl Microbiol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2022- : Oxford : Oxford University Press Original Publication: Oxford : Published for the Society for Applied Bacteriology by Blackwell Science, c1997- |
| مواضيع طبية MeSH: | Air Microbiology*, Coxiella burnetii/*isolation & purification , DNA, Bacterial/*isolation & purification , Q Fever/*veterinary, Aerosols ; Agriculture ; Animals ; Disease Outbreaks ; Environmental Monitoring/methods ; Goats ; Incidence ; Netherlands/epidemiology ; Polymerase Chain Reaction ; Q Fever/epidemiology ; Sheep, Domestic |
| مستخلص: | Aim: To investigate the Coxiella burnetii DNA content in environmental samples that may contribute to the transmission of C. burnetii. Methods and Results: During a large Q fever outbreak in the Netherlands, surface swabs and aerosol samples were collected inside stables and around six Q fever-affected ruminant farms, which are located in municipalities varying in Q fever incidence. After the outbreak in 2010, aerosol samples were collected in the same geographical areas. The use of an optimized multiplex qPCR for the detection of C. burnetii DNA revealed that all samples obtained inside stables were positive. In addition, the C. burnetii DNA content in aerosol samples collected in stables is significantly higher than in aerosol samples collected around the farms. Finally, the C. burnetii DNA content in aerosol samples collected in the same geographical locations was lower in 2010 in comparison with 2009. Conclusions: The reduction in C. burnetii DNA content in aerosol samples between 2009 and 2010 is in agreement with the reduction in Q fever incidence in the same geographical areas. Significance and Impact of the Study: The presence of C. burnetii DNA in environmental samples collected on and around ruminant farms supports the hypothesis that C. burnetii can be disseminated from ruminant farms to the surrounding areas. (© 2013 National Institute for Public Health © 2013 The Society for Applied Microbiology.) |
| المشرفين على المادة: | 0 (Aerosols) 0 (DNA, Bacterial) |
| تواريخ الأحداث: | Date Created: 20130213 Date Completed: 20140213 Latest Revision: 20221222 |
| رمز التحديث: | 20240829 |
| DOI: | 10.1111/jam.12163 |
| PMID: | 23398323 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1365-2672 |
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| DOI: | 10.1111/jam.12163 |