دورية أكاديمية
Screening of recombinant proteins as antigens in indirect ELISA for diagnosis of bovine tuberculosis.
| العنوان: | Screening of recombinant proteins as antigens in indirect ELISA for diagnosis of bovine tuberculosis. |
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| المؤلفون: | Souza II; Pós Graduação em Ciência Animal, Universidade Federal de Mato Grosso do Sul, Campo Grande, MS Brazil., Melo ES, Ramos CA, Farias TA, Osório AL, Jorge KS, Vidal CE, Silva AS, Silva MR, Pellegrin AO, Araújo FR |
| المصدر: | SpringerPlus [Springerplus] 2012 Dec; Vol. 1 (1), pp. 77. Date of Electronic Publication: 2012 Dec 22. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: SpringerPlus Country of Publication: Switzerland NLM ID: 101597967 Publication Model: Print-Electronic Cited Medium: Print ISSN: 2193-1801 (Print) Linking ISSN: 21931801 NLM ISO Abbreviation: Springerplus Subsets: PubMed not MEDLINE |
| أسماء مطبوعة: | Original Publication: Switzerland : SpringerPlus |
| مستخلص: | Bovine tuberculosis is an important infectious disease caused by Mycobacterium bovis, which is responsible for considerable economic losses. This disease constitutes a serious public health problem. Control programs in most countries, including Brazil, are based on the identification and slaughter of infected animals, as defined by the skin tuberculin test, which has its constraints. In the present study, the recombinant proteins CFP-10, ESAT-6, Mb0143, MPB83, PE5, PE13, TB10.4, TB15.3 and a chimera of ESAT-6/MPB70/MPB83 (fusion protein) were tested as ELISA antigens for the diagnosis of bovine tuberculosis. The proteins were produced in Escherichia coli, purified and tested in ELISAs with sera from 126 cattle having tested negative in the comparative intradermal tuberculin test (CITT) and 107 sera from cattle having tested positive in the CITT. Also, 236 sera from two BTB-free beef cattle herds were tested. Among the proteins tested, only the ESAT-6/MPB70/MPB83 chimera demonstrated satisfactory agreement with the CITT (kappa index: 0.688), reflecting in 83.2% sensitivity and 86.5% specificity. The ELISA absorbances of the cattle sera from BTB-free herds showed similar levels to those of CITT positive cattle, probably as the result of successive skin tuberculinizations to define the BTB-free status of the herds. However, the ELISA with the ESAT-6/MPB70/MPB83 chimera was useful to discriminate BTB positive and negative cattle in herds prior to the tuberculin skin test. |
| References: | Infect Immun. 1998 Nov;66(11):5344-9. (PMID: 9784542) Res Vet Sci. 2009 Jun;86(3):371-2. (PMID: 18930508) N Z Vet J. 1995 Jun;43(3):91-5. (PMID: 16031820) Electrophoresis. 2003 Oct;24(19-20):3405-20. (PMID: 14595687) Proc Natl Acad Sci U S A. 2003 Jun 24;100(13):7877-82. (PMID: 12788972) Tuberculosis (Edinb). 2007 May;87(3):212-7. (PMID: 17023217) Vet Microbiol. 1994 May;40(1-2):111-24. (PMID: 8073619) Vet Microbiol. 2002 Apr 2;85(4):379-89. (PMID: 11856587) J Clin Microbiol. 1990 May;28(5):913-21. (PMID: 2191012) J Clin Microbiol. 2010 Mar;48(3):791-6. (PMID: 20053861) Vet Parasitol. 2005 Nov 5;133(4):369-70. (PMID: 16011877) J Clin Microbiol. 2006 Dec;44(12):4326-35. (PMID: 17005738) J Immunol Methods. 2002 Dec 1;270(1):19-26. (PMID: 12379335) Microbiology (Reading). 1995 Sep;141 ( Pt 9):2131-8. (PMID: 7496524) Semin Nucl Med. 1978 Oct;8(4):283-98. (PMID: 112681) Vet Rec. 2006 Apr 1;158(13):429-32. (PMID: 16581992) Clin Vaccine Immunol. 2008 Dec;15(12):1834-8. (PMID: 18927068) Clin Chem. 1993 Apr;39(4):561-77. (PMID: 8472349) J Vet Diagn Invest. 2011 May;23(3):499-503. (PMID: 21908278) Vet Microbiol. 1998 Aug 15;62(4):281-90. (PMID: 9791874) J Vet Med B Infect Dis Vet Public Health. 2001 Apr;48(3):161-6. (PMID: 11393811) Prev Vet Med. 2011 Feb 1;98(2-3):142-51. (PMID: 21122932) Clin Vaccine Immunol. 2006 Jun;13(6):648-54. (PMID: 16760322) Clin Vaccine Immunol. 2011 Nov;18(11):1882-8. (PMID: 21918115) Clin Vaccine Immunol. 2009 Sep;16(9):1352-9. (PMID: 19641100) Vet Microbiol. 1992 Mar;30(4):343-54. (PMID: 1585626) J Clin Microbiol. 2003 Aug;41(8):3719-28. (PMID: 12904381) Am J Respir Crit Care Med. 1999 Jan;159(1):15-21. (PMID: 9872812) Can Vet J. 2009 Nov;50(11):1169-76. (PMID: 20119541) |
| فهرسة مساهمة: | Keywords: Cattle; ELISA; Mycobacterium bovis; Recombinant proteins; Serology |
| تواريخ الأحداث: | Date Created: 20130220 Date Completed: 20141126 Latest Revision: 20211021 |
| رمز التحديث: | 20240628 |
| مُعرف محوري في PubMed: | PMC3569591 |
| DOI: | 10.1186/2193-1801-1-77 |
| PMID: | 23419946 |
| قاعدة البيانات: | MEDLINE |
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Full Text Finder | تدمد: | 2193-1801 |
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| DOI: | 10.1186/2193-1801-1-77 |