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Identification of thermophilic bacterial strains producing thermotolerant hydrolytic enzymes from manure compost.

التفاصيل البيبلوغرافية
العنوان: Identification of thermophilic bacterial strains producing thermotolerant hydrolytic enzymes from manure compost.
المؤلفون: Charbonneau DM; Département de Chimie-Biologie, Université du Québec à Trois-Rivières, 3351 Boul. Des Forges, C.P. 500, Trois-Rivières (Québec), G9A 5H7 Canada., Meddeb-Mouelhi F, Boissinot M, Sirois M, Beauregard M
المصدر: Indian journal of microbiology [Indian J Microbiol] 2012 Mar; Vol. 52 (1), pp. 41-7. Date of Electronic Publication: 2011 Feb 03.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Springer India Country of Publication: India NLM ID: 0374703 Publication Model: Print-Electronic Cited Medium: Print ISSN: 0046-8991 (Print) Linking ISSN: 00468991 NLM ISO Abbreviation: Indian J Microbiol Subsets: PubMed not MEDLINE
أسماء مطبوعة: Publication: New Delhi : Springer India
Original Publication: Calcutta, Association of Microbiologists of India.
مستخلص: Ten thermophilic bacterial strains were isolated from manure compost. Phylogenetic analysis based on 16S rRNA genes and biochemical characterization allowed identification of four different species belonging to four genera: Geobacillus thermodenitrificans, Bacillus smithii, Ureibacillus suwonensis and Aneurinibacillus thermoaerophilus. PCR-RFLP profiles of the 16S-ITS-23S rRNA region allowed us to distinguish two subgroups among the G. thermodenitrificans isolates. Isolates were screened for thermotolerant hydrolytic activities (60-65°C). Thermotolerant lipolytic activities were detected for G. thermodenitrificans, A. thermoaerophilus and B. smithii. Thermotolerant protease, α-amylase and xylanase activities were also observed in the G. thermodenitrificans group. These species represent a source of potential novel thermostable enzymes for industrial applications.
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فهرسة مساهمة: Keywords: 16S rDNA; Hydrolytic enzymes; Manure compost; Thermal stability; Thermophilic bacteria
تواريخ الأحداث: Date Created: 20130302 Date Completed: 20130501 Latest Revision: 20211021
رمز التحديث: 20221213
مُعرف محوري في PubMed: PMC3298584
DOI: 10.1007/s12088-011-0156-8
PMID: 23448754
قاعدة البيانات: MEDLINE
الوصف
تدمد:0046-8991
DOI:10.1007/s12088-011-0156-8