دورية أكاديمية
Development of amplified fragment length polymorphism (AFLP)-derived specific primer for the detection of Fusarium solani aetiological agent of peanut brown root rot.
| العنوان: | Development of amplified fragment length polymorphism (AFLP)-derived specific primer for the detection of Fusarium solani aetiological agent of peanut brown root rot. |
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| المؤلفون: | Casasnovas F; Fellowship CONICET, Buenos Aires, Argentina., Fantini EN, Palazzini JM, Giaj-Merlera G, Chulze SN, Reynoso MM, Torres AM |
| المصدر: | Journal of applied microbiology [J Appl Microbiol] 2013 Jun; Vol. 114 (6), pp. 1782-92. Date of Electronic Publication: 2013 Mar 25. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Oxford University Press Country of Publication: England NLM ID: 9706280 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1365-2672 (Electronic) Linking ISSN: 13645072 NLM ISO Abbreviation: J Appl Microbiol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2022- : Oxford : Oxford University Press Original Publication: Oxford : Published for the Society for Applied Bacteriology by Blackwell Science, c1997- |
| مواضيع طبية MeSH: | Amplified Fragment Length Polymorphism Analysis*, DNA Primers/*chemistry , Fusarium/*isolation & purification, Arachis/microbiology ; DNA, Fungal/chemistry ; Fusarium/classification ; Fusarium/genetics ; Soil Microbiology |
| مستخلص: | Aims: The objective of this work was to design an amplified fragment length polymorphism (AFLP)-derived specific primer for the detection of Fusarium solani aetiological agent of peanut brown root rot (PBRR) in plant material and soil. Methods and Results: Specific primers for the detection of the pathogen were designed based on an amplified region using AFLPs. The banding patterns by AFLPs showed that isolates from diseased roots were clearly distinguishable from others members of the F. solani species complex. Many bands were specific to F. solani PBRR, one of these fragments was selected and sequenced. Sequence obtained was used to develop specific PCR primers for the identification of pathogen in pure culture and in plant material and soil. Primer pair FS1/FS2 amplified a single DNA product of 175 bp. Other fungal isolates occurring in soil, included F. solani non-PBRR, were not detected by these specific primers. The assay was effective for the detection of pathogen from diseased root and infected soils. Conclusions: The designed primers for F. solani causing PBRR can be used in a PCR diagnostic protocol to rapidly and reliably detect and identify this pathogen. Significance and Impact of the Study: These diagnostic PCR primers will aid the detection of F. solani causing PBRR in diseased root and natural infected soils. The method developed could be a helpful tool for epidemiological studies and to avoid the spread of this serious disease in new areas. (© 2013 The Society for Applied Microbiology.) |
| المشرفين على المادة: | 0 (DNA Primers) 0 (DNA, Fungal) |
| تواريخ الأحداث: | Date Created: 20130312 Date Completed: 20140128 Latest Revision: 20181202 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1111/jam.12183 |
| PMID: | 23472596 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1365-2672 |
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| DOI: | 10.1111/jam.12183 |