دورية أكاديمية

The expression of mucin genes and the presence of mucin gene products in the equine endometrium.

التفاصيل البيبلوغرافية
العنوان: The expression of mucin genes and the presence of mucin gene products in the equine endometrium.
المؤلفون: Maischberger E; School of Veterinary Medicine, Veterinary Sciences Centre, University College Dublin, Belfield, Dublin 4, Ireland., Cummins CA, Fitzpatrick E, Gallagher ME, Worrall S, Rousseau K, Thornton DJ, Meijer WG, Miranda-Casoluengo R, Duggan VE, Carrington SD, Irwin JA, Reid CJ
المصدر: Research in veterinary science [Res Vet Sci] 2013 Aug; Vol. 95 (1), pp. 169-75. Date of Electronic Publication: 2013 Apr 11.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: British Veterinary Association Country of Publication: England NLM ID: 0401300 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1532-2661 (Electronic) Linking ISSN: 00345288 NLM ISO Abbreviation: Res Vet Sci Subsets: MEDLINE
أسماء مطبوعة: Publication: London : British Veterinary Association
Original Publication: Oxford.
مواضيع طبية MeSH: Endometrium/*metabolism , Estrous Cycle/*metabolism , Horses/*metabolism , Mucins/*biosynthesis, Animals ; Blotting, Western/veterinary ; Estrous Cycle/genetics ; Female ; Gene Expression ; Horses/genetics ; In Situ Hybridization/veterinary ; Mucins/genetics ; Mucins/metabolism ; RNA/chemistry ; RNA/genetics ; Reverse Transcriptase Polymerase Chain Reaction/veterinary
مستخلص: In the equine reproductive tract, little is known about mucin gene expression and the role of mucins in barrier function and host-cell interaction. The aims of the study were to identify equine orthologs of mammalian mucin genes using available equine sequence data, to profile expression of equine orthologous mucin genes in the endometrium using reverse transcriptase polymerase chain reaction (RT-PCR), to determine spatial expression patterns of mucin genes using in situ hybridisation, and to confirm the presence of mucin gene products using Western blotting and equine-specific mucin antibodies during oestrus and dioestrus. While the mucin gene expression pattern in equine endometrium is similar to that of other mammals, several mucins appear to be uniquely expressed in this tissue (eqMUC3B, 7, 18, and 20) and one is hormonally regulated (eqMUC3B).
(Copyright © 2013 Elsevier Ltd. All rights reserved.)
المشرفين على المادة: 0 (Mucins)
63231-63-0 (RNA)
تواريخ الأحداث: Date Created: 20130416 Date Completed: 20140220 Latest Revision: 20130520
رمز التحديث: 20221213
DOI: 10.1016/j.rvsc.2013.03.012
PMID: 23583093
قاعدة البيانات: MEDLINE
الوصف
تدمد:1532-2661
DOI:10.1016/j.rvsc.2013.03.012