دورية أكاديمية
Gene expression study related with the intrinsic pathway of apoptosis in bladder cancer by real-time PCR technique.
| العنوان: | Gene expression study related with the intrinsic pathway of apoptosis in bladder cancer by real-time PCR technique. |
|---|---|
| المؤلفون: | Barione DF; Departamento de Cirurgia e Anatomia, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP, Brasil., Lizarte FS, Novais PC, de Carvalho CA, Valeri FC, Peria FM, de Oliveira HF, Zanette DL, Silva WA Jr, Cologna AJ, Reis RB, Tucci S Jr, Martins AC, Tirapelli DP, Tirapelli LF |
| المصدر: | Genetics and molecular research : GMR [Genet Mol Res] 2013 Apr 02; Vol. 12 (2), pp. 878-86. Date of Electronic Publication: 2013 Apr 02. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: FUNPEC Country of Publication: Brazil NLM ID: 101169387 Publication Model: Electronic Cited Medium: Internet ISSN: 1676-5680 (Electronic) Linking ISSN: 16765680 NLM ISO Abbreviation: Genet Mol Res Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: [Ribeirao Preto, SP Brazil] : FUNPEC, 2002- |
| مواضيع طبية MeSH: | Gene Expression Regulation, Neoplastic* , Signal Transduction*, Apoptosis/*genetics , Urinary Bladder Neoplasms/*genetics , Urinary Bladder Neoplasms/*metabolism, Apoptotic Protease-Activating Factor 1/genetics ; Apoptotic Protease-Activating Factor 1/metabolism ; Caspase 9/genetics ; Caspase 9/metabolism ; Cytochromes c/genetics ; Cytochromes c/metabolism ; Gene Expression Profiling ; Humans ; Proto-Oncogene Proteins c-bcl-2/genetics ; Proto-Oncogene Proteins c-bcl-2/metabolism ; Real-Time Polymerase Chain Reaction |
| مستخلص: | We examined the expression of anti-apoptotic genes (XIAP and Bcl-2) and apoptotic genes (cytochrome c, caspase-9, Apaf-1) in tissue samples of patients with superficial bladder cancer. Thirty-two bladder cancer tissue samples (8 papillary urothelial neoplasm of low malignant potential, 10 low-grade, and 14 high-grade) and 8 normal bladder tissue samples from necropsy were used for the study of gene expression by real-time PCR analysis. Analysis of the expression of apoptotic gene constituents of an apoptosome demonstrated an increase in Apaf-1 expression in the three tumor grades when compared with the control (P < 0.01, P < 0.05, and P < 0.01), low expression of caspase-9 in all groups (P < 0.05), and an increase in cytochrome c expression in all tumor grades in relation to the control, although without statistically significant difference. The expression of anti-apoptotic genes revealed an increase in XIAP expression in all tumor grades in relation to the control, although without statistically significant difference, and low expression of Bcl-2 in all tumor grades and the control (P < 0.05). The results proved that there is low evidence of apoptotic activity by the intrinsic pathway, demonstrated by the low expression of caspase-9 and considerable increase in XIAP expression, which may render these genes potential therapeutic targets in bladder cancer treatment. |
| المشرفين على المادة: | 0 (Apoptotic Protease-Activating Factor 1) 0 (Proto-Oncogene Proteins c-bcl-2) 9007-43-6 (Cytochromes c) EC 3.4.22.- (Caspase 9) |
| تواريخ الأحداث: | Date Created: 20130425 Date Completed: 20131022 Latest Revision: 20130424 |
| رمز التحديث: | 20231215 |
| DOI: | 10.4238/2013.April.2.4 |
| PMID: | 23613234 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1676-5680 |
|---|---|
| DOI: | 10.4238/2013.April.2.4 |