دورية أكاديمية
أعرض في EDS

Impact of agr dysfunction on virulence profiles and infections associated with a novel methicillin-resistant Staphylococcus aureus (MRSA) variant of the lineage ST1-SCCmec IV.

التفاصيل البيبلوغرافية
العنوان: Impact of agr dysfunction on virulence profiles and infections associated with a novel methicillin-resistant Staphylococcus aureus (MRSA) variant of the lineage ST1-SCCmec IV.
المؤلفون: Ferreira FA; Departamento de Microbiologia Médica, Instituto de Microbiologia Paulo de Góes, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brazil., Souza RR, de Sousa Moraes B, de Amorim Ferreira AM, Américo MA, Fracalanzza SE, Dos Santos Silva Couceiro JN, Sá Figueiredo AM
المصدر: BMC microbiology [BMC Microbiol] 2013 Apr 27; Vol. 13, pp. 93. Date of Electronic Publication: 2013 Apr 27.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: BioMed Central Country of Publication: England NLM ID: 100966981 Publication Model: Electronic Cited Medium: Internet ISSN: 1471-2180 (Electronic) Linking ISSN: 14712180 NLM ISO Abbreviation: BMC Microbiol Subsets: MEDLINE
أسماء مطبوعة: Original Publication: London : BioMed Central, [2001-
مواضيع طبية MeSH: Biofilms/*growth & development , Catheter-Related Infections/*microbiology , Methicillin-Resistant Staphylococcus aureus/*pathogenicity , Methicillin-Resistant Staphylococcus aureus/*physiology , Staphylococcal Infections/*microbiology , Trans-Activators/*deficiency, Animals ; Bacterial Adhesion ; Bacterial Proteins ; Brazil ; Disease Models, Animal ; Endocytosis ; Genotype ; Humans ; Male ; Methicillin-Resistant Staphylococcus aureus/classification ; Methicillin-Resistant Staphylococcus aureus/isolation & purification ; Mice ; Mice, Inbred BALB C ; Molecular Typing ; Virulence
مستخلص: Background: A novel variant of the ST1-SCCmecIV methicillin-resistant Staphylococcus aureus (MRSA) lineage, mostly associated with nosocomial bloodstream infections (BSI), has emerged in Rio de Janeiro. Bacterial biofilm has been considered a major virulence factor in central venous catheter-associated BSI. The mechanisms involved in biofilm formation/accumulation are multifactorial and complex. Studies have suggested that biofilm production was affected in vitro and vivo for agr-null mutants of S. aureus.
Results: The impact of naturally occurring inhibition of agr signaling on virulence profiles and infections associated with the ST1 variant was investigated. agr dysfunction was detected in a significant percentage (13%) of the isolates with concomitant increase in biofilm accumulation in vitro and in vivo, and enhanced ability to adhere to and invade airway cells. The biofilm formed by these ST1 isolates was ica-independent and proteinaceous in nature. In fact, the improved colonization properties were paralleled by an increased expression of the biofilm-associated genes fnbA, spa and sasG. The transcription of sarA, a positive regulator of agr, was two-times reduced for the agr-dysfunctional MRSA. Remarkably, the agr inhibition was genetically stable. Indeed, agr-dysfunctional isolates succeed to colonize and cause both acute and chronic infections in hospitalized patients, and also to effectively accumulate biofilm in a mouse subcutaneous catheter implant model.
Conclusion: The ability of agr-dysfunctional isolates to cause infections in humans and to form biofilm in the animal model suggests that therapeutic approaches based on agr-inactivation strategies are unlikely to be effective in controlling human-device infections caused by ST1 isolates. The increased biofilm accumulation associated with the acquisition of multiple antimicrobial resistant traits might have influenced (at least in part) the expansion of this USA400 related clone in our hospitals.
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المشرفين على المادة: 0 (Agr protein, Staphylococcus aureus)
0 (Bacterial Proteins)
0 (Trans-Activators)
تواريخ الأحداث: Date Created: 20130430 Date Completed: 20131022 Latest Revision: 20211021
رمز التحديث: 20240628
مُعرف محوري في PubMed: PMC3652751
DOI: 10.1186/1471-2180-13-93
PMID: 23622558
قاعدة البيانات: MEDLINE
الوصف
تدمد:1471-2180
DOI:10.1186/1471-2180-13-93