دورية أكاديمية
Analysis of amino acids in tobacco by derivatization and dispersive liquid-liquid microextraction based on solidification of floating organic droplet method.
| العنوان: | Analysis of amino acids in tobacco by derivatization and dispersive liquid-liquid microextraction based on solidification of floating organic droplet method. |
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| المؤلفون: | Li G; Technology Center, Shanghai Tobacco Group Co., Ltd., 200082 Shanghai, China., Wu D, Xie W, Sha Y, Lin H, Liu B |
| المصدر: | Journal of chromatography. A [J Chromatogr A] 2013 Jun 28; Vol. 1296, pp. 243-7. Date of Electronic Publication: 2013 Apr 08. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Country of Publication: Netherlands NLM ID: 9318488 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-3778 (Electronic) Linking ISSN: 00219673 NLM ISO Abbreviation: J Chromatogr A Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Amsterdam ; New York : Elsevier, 1993- |
| مواضيع طبية MeSH: | Amino Acids/*analysis , Liquid Phase Microextraction/*methods , Nicotiana/*chemistry, Acetone/chemistry ; Calibration ; Dodecanol/chemistry ; Gas Chromatography-Mass Spectrometry ; Limit of Detection ; Plant Leaves/chemistry ; Reproducibility of Results ; Temperature |
| مستخلص: | A new one-step derivatization and microextraction technique was developed for determination of amino acids in tobacco samples. In the proposed method, amino acids were derivatized with isobutyl chloroformate (IBCF) in aqueous solution. The derivatives were extracted by dispersive liquid-liquid microextraction based on solidification of floating organic droplet (DLLME-SFO). Parameters affecting the extraction efficiency were investigated in detail. The optimum conditions were as follows: a mixture of 500μL acetone, containing 40μL 2-dodecanol, was rapidly injected by syringe into the 2mL water sample. After centrifugation, the 2-dodecanol droplets were floated at the top of the tube. Then, the tube was cooled in an ice bath. After 5min the solvent had solidified and was then transferred into a conical vial; it melted quickly at room temperature and it was injected into a gas chromatograph for analysis. Under the optimum conditions, the limits of detection were of the order of 0.12-2.82μg/mL. The calibration curves showed good linearity over the investigated concentration range between 0.5 and 200μg/mL with a coefficient of estimation (R(2))>0.9887 for GC-MS (SIM). The proposed method is an alternative approach to the quantification of amino acids in tobacco samples. (Copyright © 2013 Elsevier B.V. All rights reserved.) |
| المشرفين على المادة: | 0 (Amino Acids) 1364PS73AF (Acetone) 178A96NLP2 (Dodecanol) |
| تواريخ الأحداث: | Date Created: 20130430 Date Completed: 20131230 Latest Revision: 20231213 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1016/j.chroma.2013.03.076 |
| PMID: | 23623365 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1873-3778 |
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| DOI: | 10.1016/j.chroma.2013.03.076 |