دورية أكاديمية
The succinoglycan endoglycanase encoded by exoK is required for efficient symbiosis of Sinorhizobium meliloti 1021 with the host plants Medicago truncatula and Medicago sativa (Alfalfa).
| العنوان: | The succinoglycan endoglycanase encoded by exoK is required for efficient symbiosis of Sinorhizobium meliloti 1021 with the host plants Medicago truncatula and Medicago sativa (Alfalfa). |
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| المؤلفون: | Mendis HC; Department of Biological Science, Florida State University, Tallahassee, FL 32306-4370, USA., Queiroux C, Brewer TE, Davis OM, Washburn BK, Jones KM |
| المصدر: | Molecular plant-microbe interactions : MPMI [Mol Plant Microbe Interact] 2013 Sep; Vol. 26 (9), pp. 1089-105. |
| نوع المنشور: | Journal Article; Research Support, U.S. Gov't, Non-P.H.S. |
| اللغة: | English |
| بيانات الدورية: | Publisher: APS Press Country of Publication: United States NLM ID: 9107902 Publication Model: Print Cited Medium: Print ISSN: 0894-0282 (Print) Linking ISSN: 08940282 NLM ISO Abbreviation: Mol Plant Microbe Interact Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: St. Paul, MN : APS Press, [c1987- |
| مواضيع طبية MeSH: | Symbiosis*, Glycoside Hydrolases/*genetics , Medicago sativa/*microbiology , Medicago truncatula/*microbiology , Sinorhizobium meliloti/*enzymology, Bacterial Proteins/genetics ; Bacterial Proteins/metabolism ; Biomass ; Genetic Complementation Test ; Glycoside Hydrolases/metabolism ; Mutation ; Nitrogen Fixation ; Phenotype ; Plant Root Nodulation ; Plant Roots/microbiology ; Plant Shoots/microbiology ; Polysaccharides, Bacterial/metabolism ; Recombinant Fusion Proteins ; Sinorhizobium meliloti/genetics ; Sinorhizobium meliloti/physiology |
| مستخلص: | The acidic polysaccharide succinoglycan produced by the nitrogen-fixing rhizobial symbiont Sinorhizobium meliloti 1021 is required for this bacterium to invade the host plant Medicago truncatula and to efficiently invade the host plant M. sativa (alfalfa). The β-glucanase enzyme encoded by exoK has previously been demonstrated to cleave succinoglycan and participate in producing the low molecular weight form of this polysaccharide. Here, we show that exoK is required for efficient S. meliloti invasion of both M. truncatula and alfalfa. Deletion mutants of exoK have a substantial reduction in symbiotic productivity on both of these plant hosts. Insertion mutants of exoK have an even less productive symbiosis than the deletion mutants with the host M. truncatula that is caused by a secondary effect of the insertion itself, and may be due to a polar effect on the expression of the downstream exoLAMON genes. |
| المشرفين على المادة: | 0 (Bacterial Proteins) 0 (Polysaccharides, Bacterial) 0 (Recombinant Fusion Proteins) 73667-50-2 (succinoglycan) EC 3.2.1.- (Glycoside Hydrolases) EC 3.2.1.- (glycanase) |
| تواريخ الأحداث: | Date Created: 20130510 Date Completed: 20131114 Latest Revision: 20130802 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1094/MPMI-03-13-0087-R |
| PMID: | 23656330 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 0894-0282 |
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| DOI: | 10.1094/MPMI-03-13-0087-R |