دورية أكاديمية
Total parenteral nutrition-associated lamina propria inflammation in mice is mediated by a MyD88-dependent mechanism.
| العنوان: | Total parenteral nutrition-associated lamina propria inflammation in mice is mediated by a MyD88-dependent mechanism. |
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| المؤلفون: | Miyasaka EA; Section of Pediatric Surgery, Department of Surgery, University of Michigan, Ann Arbor, MI 48109, USA., Feng Y, Poroyko V, Falkowski NR, Erb-Downward J, Gillilland MG 3rd, Mason KL, Huffnagle GB, Teitelbaum DH |
| المصدر: | Journal of immunology (Baltimore, Md. : 1950) [J Immunol] 2013 Jun 15; Vol. 190 (12), pp. 6607-15. Date of Electronic Publication: 2013 May 10. |
| نوع المنشور: | Journal Article; Research Support, N.I.H., Extramural |
| اللغة: | English |
| بيانات الدورية: | Publisher: American Association of Immunologists Country of Publication: United States NLM ID: 2985117R Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1550-6606 (Electronic) Linking ISSN: 00221767 NLM ISO Abbreviation: J Immunol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Bethesda, MD : American Association of Immunologists Original Publication: Baltimore : Williams & Wilkins, c1950- |
| مواضيع طبية MeSH: | Inflammation/*pathology , Intestinal Mucosa/*microbiology , Intestinal Mucosa/*pathology , Myeloid Differentiation Factor 88/*immunology , Parenteral Nutrition, Total/*adverse effects, Animals ; Flow Cytometry ; Inflammation/etiology ; Inflammation/microbiology ; Intestinal Mucosa/immunology ; Male ; Mice ; Mice, Inbred C57BL ; Mice, Knockout ; Microscopy, Fluorescence ; Mucous Membrane/microbiology ; Mucous Membrane/pathology ; Polymorphism, Restriction Fragment Length ; Real-Time Polymerase Chain Reaction |
| مستخلص: | Enteral nutrient deprivation via total parenteral nutrition (TPN) administration leads to local mucosal inflammatory responses, but the underlying mechanisms are unknown. Wild-type (WT) and MyD88(-/-) mice underwent jugular vein cannulation. One group received TPN without chow, and controls received standard chow. After 7 d, we harvested intestinal mucosally associated bacteria and isolated small-bowel lamina propria (LP) cells. Bacterial populations were analyzed using 454 pyrosequencing. LP cells were analyzed using quantitative PCR and multicolor flow cytometry. WT, control mucosally associated microbiota were Firmicutes-dominant, whereas WT TPN mice were Proteobacteria-domiant. Similar changes were observed in MyD88(-/-) mice with TPN administration. UniFrac analysis showed divergent small bowel and colonic bacterial communities in controls, merging toward similar microbiota (but distinct from controls) with TPN. The percentage of LP T regulatory cells significantly decreased with TPN in WT mice. F4/80(+)CD11b(+)CD11c(dull/-) macrophage-derived proinflammatory cytokines significantly increased with TPN. These proinflammatory immunologic changes were significantly abrogated in MyD88(-/-) TPN mice. Thus, TPN administration is associated with significant expansion of Proteobacteria within the intestinal microbiota and increased proinflammatory LP cytokines. Additionally, MyD88 signaling blockade abrogated decline in epithelial cell proliferation and epithelial barrier function loss. |
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| معلومات مُعتمدة: | R01 AI044076 United States AI NIAID NIH HHS; T32 HD007505 United States HD NICHD NIH HHS; R01 AI-44076-14 United States AI NIAID NIH HHS; T-32HD007505 United States HD NICHD NIH HHS |
| المشرفين على المادة: | 0 (Myd88 protein, mouse) 0 (Myeloid Differentiation Factor 88) |
| تواريخ الأحداث: | Date Created: 20130514 Date Completed: 20130812 Latest Revision: 20211021 |
| رمز التحديث: | 20231215 |
| مُعرف محوري في PubMed: | PMC3679213 |
| DOI: | 10.4049/jimmunol.1201746 |
| PMID: | 23667106 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1550-6606 |
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| DOI: | 10.4049/jimmunol.1201746 |