دورية أكاديمية
[Effect and mechanism of huatuo zaizao extractum on focal cerebral ischemia/reperfusion-induced blood-brain barrier injury in rats].
| العنوان: | [Effect and mechanism of huatuo zaizao extractum on focal cerebral ischemia/reperfusion-induced blood-brain barrier injury in rats]. |
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| المؤلفون: | Zheng YQ; Xiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing 100091, China., Yao MJ, Liu JX, Song WT, Li L, Liu SB, Hu Y, Si JX |
| المصدر: | Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica [Zhongguo Zhong Yao Za Zhi] 2013 Feb; Vol. 38 (4), pp. 585-90. |
| نوع المنشور: | Journal Article |
| اللغة: | Chinese |
| بيانات الدورية: | Publisher: Zhongguo yao xue hui : Zhongguo Zhong yi yan jiu yuan Zhong yao yan jiu suo Country of Publication: China NLM ID: 8913656 Publication Model: Print Cited Medium: Print ISSN: 1001-5302 (Print) Linking ISSN: 10015302 NLM ISO Abbreviation: Zhongguo Zhong Yao Za Zhi Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Beijing : Zhongguo yao xue hui : Zhongguo Zhong yi yan jiu yuan Zhong yao yan jiu suo Original Publication: [Beijing] : Zhongguo yao xue hui : [Zhongguo Zhong yi yan jiu yuan Zhong yao yan jiu suo, 1989- |
| مواضيع طبية MeSH: | Blood-Brain Barrier/*drug effects , Blood-Brain Barrier/*injuries , Drugs, Chinese Herbal/*pharmacology , Infarction, Middle Cerebral Artery/*complications , Reperfusion Injury/*complications, Animals ; Behavior, Animal/drug effects ; Behavior, Animal/physiology ; Dose-Response Relationship, Drug ; G-Protein-Coupled Receptor Kinase 2/metabolism ; Gene Expression Regulation, Enzymologic/drug effects ; Male ; Matrix Metalloproteinase 2/metabolism ; Matrix Metalloproteinase 9/metabolism ; Microvessels/drug effects ; Rats ; Rats, Sprague-Dawley ; Reperfusion Injury/metabolism ; Reperfusion Injury/physiopathology |
| مستخلص: | Objective: To observe the effect and mechanism of Huatuo Zaizao extractum (HTZZ) on focal ischemia/reperfusion (I/R) blood-brain barrier injury induced by middle cerebral artery occlusion. Method: Sixty healthy male adult Sprague-Dawley rats was randomly divided into the sham operation group, the MCAO model group, the Tanakan (20 mg x kg(-1)) group, and high, middle and low-dose HTZZ groups (5, 2.5, 1.25 g x kg(-1)), with 10 in each group and single-dose duodenal administration. Middle cerebral artery occlusion was adopted to establish the rat focal I/R model. After ischemia for 90 min and reperfusion for 24 h, the pathological injury at the ischemia side was observed by HE staining. The blood-brain barrier structure was observed under transmission electron microscope. Expressions of G protein-coupled receptor kinases 2 (GRK2), matrix metalloproteinases 2 (MMP-2) and MMP-9 were detected by western blotting technique. Result: After 90 min MCAO/24 h reperfusion, penumbra cerebral cortical micro-vessels showed edema, mitochondrial injury, vacuolation, membrane injury and reduction. Along with the changes, sub-cells of G protein-coupled receptor kinase 2 (GRK2) in cortical penumbra brain tissues transferred from cytoplasm to membrane, with increase in expressions of MMP-2 and MMP-9. HTZZ could effectively recover cerebral micro-vascular endothelial edemaand blood-brain barrier ultrastructure injury induced by I/R, reduce expression of functional (membrane coupling) GRK2, and inhibit expressions of MMP-2 and MMP-9. Conclusion: Cell membrane coupling GRK2 may be the effective target of Huatuo Zaizao extractum. |
| المشرفين على المادة: | 0 (Drugs, Chinese Herbal) EC 2.7.11.15 (Grk2 protein, rat) EC 2.7.11.16 (G-Protein-Coupled Receptor Kinase 2) EC 3.4.24.24 (Matrix Metalloproteinase 2) EC 3.4.24.35 (Matrix Metalloproteinase 9) |
| تواريخ الأحداث: | Date Created: 20130530 Date Completed: 20130620 Latest Revision: 20191210 |
| رمز التحديث: | 20240628 |
| PMID: | 23713288 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1001-5302 |
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