دورية أكاديمية
Adverse effect of demineralized bone powder on osteogenesis of human mesenchymal stem cells.
| العنوان: | Adverse effect of demineralized bone powder on osteogenesis of human mesenchymal stem cells. |
|---|---|
| المؤلفون: | Pflum ZE; Department of Orthopedics and Rehabilitation, University of Wisconsin-Madison, Madison, WI, United States of America., Palumbo SL; Department of Orthopedics and Rehabilitation, University of Wisconsin-Madison, Madison, WI, United States of America., Li WJ; Department of Orthopedics and Rehabilitation, University of Wisconsin-Madison, Madison, WI, United States of America; Department of Biomedical Engineering, University of Wisconsin-Madison, Madison, WI, United States of America. Electronic address: li@ortho.wisc.edu. |
| المصدر: | Experimental cell research [Exp Cell Res] 2013 Aug 01; Vol. 319 (13), pp. 1942-1955. Date of Electronic Publication: 2013 May 28. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Academic Press Country of Publication: United States NLM ID: 0373226 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1090-2422 (Electronic) Linking ISSN: 00144827 NLM ISO Abbreviation: Exp Cell Res Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Orlando Fl : Academic Press Original Publication: New York, Academic Press. |
| مواضيع طبية MeSH: | Bone Substitutes/*adverse effects , Bone and Bones/*chemistry , Mesenchymal Stem Cells/*drug effects , Osteogenesis/*drug effects, Adult ; Angiostatins/metabolism ; Bone Demineralization Technique ; Cells, Cultured ; Female ; Humans ; Insulin-Like Growth Factor Binding Protein 1/metabolism ; Male ; Mesenchymal Stem Cells/cytology ; Middle Aged ; Powders |
| مستخلص: | Demineralized bone powder (DBP) has been used by clinicians for years to treat bone defects. Although DBP treatment often leads to successful bone healing, a number of studies using DBP have demonstrated poor bone formation. It is known that soluble factors released from DBP modulate bone formation. We hypothesized that DBP releases or interacts with soluble factors that modulate osteogenesis of mesenchymal stem cells (MSCs). Our in vitro study demonstrated that the expression of mRNA transcripts of bone-related markers decreased in osteogenic culture of human MSCs (hMSCs) with DBP compared to that without DBP. Using a high-throughput protein array, we identified insulin-like growth factor binding protein-1, thrombospondin, and angiostatin that were found abundant in the medium cultured with DBP. Separately, we detected a significant reduction of soluble calcium and phosphate in the DBP-present medium compared to that in the DBP-absent medium, and showed that hMSC osteogenesis was regulated by the amounts of soluble calcium and phosphate in the medium. Moreover, DBP was shown to sequester soluble calcium and phosphate in the medium, thereby depleting them from interacting with hMSCs during osteogenesis. This study provides a possible explanation to an important question associated with the use of DBP in clinical treatments. (Copyright © 2013 Elsevier Inc. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Calcium; Demineralized bone powder; Mesenchymal stem cell; Osteogenesis; Phosphate |
| المشرفين على المادة: | 0 (Bone Substitutes) 0 (IGFBP1 protein, human) 0 (Insulin-Like Growth Factor Binding Protein 1) 0 (Powders) 86090-08-6 (Angiostatins) |
| تواريخ الأحداث: | Date Created: 20130604 Date Completed: 20130919 Latest Revision: 20200510 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1016/j.yexcr.2013.05.020 |
| PMID: | 23726837 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1090-2422 |
|---|---|
| DOI: | 10.1016/j.yexcr.2013.05.020 |