دورية أكاديمية
GABA(A) receptor characterization and subunit localization in the human sub-ventricular zone.
| العنوان: | GABA(A) receptor characterization and subunit localization in the human sub-ventricular zone. |
|---|---|
| المؤلفون: | Dieriks BV; Department of Anatomy with Radiology and Centre for Brain Research, Faculty of Medical and Health Science, University of Auckland, Private Bag 92019, Auckland, New Zealand., Waldvogel HJ, Monzo HJ, Faull RL, Curtis MA |
| المصدر: | Journal of chemical neuroanatomy [J Chem Neuroanat] 2013 Sep; Vol. 52, pp. 58-68. Date of Electronic Publication: 2013 Jun 11. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Science Publisher Country of Publication: Netherlands NLM ID: 8902615 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-6300 (Electronic) Linking ISSN: 08910618 NLM ISO Abbreviation: J Chem Neuroanat Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Amsterdam : Elsevier Science Publisher Original Publication: Chichester, Sussex, England : Wiley & Sons, c1988- |
| مواضيع طبية MeSH: | Cerebral Ventricles/*chemistry , Neural Stem Cells/*chemistry , Protein Subunits/*metabolism , Receptors, GABA-A/*metabolism, Adult ; Aged ; Cell Proliferation ; Cerebral Ventricles/cytology ; Cerebral Ventricles/metabolism ; Female ; Humans ; Male ; Middle Aged ; Neural Stem Cells/metabolism ; Protein Subunits/biosynthesis ; Receptors, GABA-A/biosynthesis |
| مستخلص: | It is now well established that the human brain continuously produces new stem cells until well into old age. One of these stem-cell rich areas in the human brain is the sub-ventricular zone (SVZ). The human SVZ is organized in four distinctive layers containing type A, B and C cells. To date, no studies have investigated the distribution of inhibitory neurotransmitters such as γ-aminobutyric acid (GABA) and their respective receptors on the different cell types in the human SVZ. GABA(A) receptors (GABA(A)R) are ubiquitously expressed, inhibitory heteropentameric chloride ion channels comprised of a variety of subunits that are targeted by many prescribed drugs. In this study we present detailed immunohistochemical data on the regional and cellular localization of α₁, α₂, α3, β₂,₃ and γ₂ subunits of GABA(A)R in the human SVZ. The results from our double and triple labeling studies demonstrate that the cell types and subunit composition throughout the SVZ is heterogeneous; the thickness of the SVZ and GABA(A)R α₂ and γ₂ expression is increased especially in the vicinity of large SVZ blood vessels. GABA(A)R γ₂ is the most specific to the SVZ and present on various cells that express, either glial fibrillary acidic protein (GFAPδ) or polysialic acid-neural cell adhesion molecule (PSA-NCAM) separately, or together in a respective ratio of 7:6:2. Proliferating (type C) cells in the SVZ express GAD65/67, GFAPδ and GABA(A)R β₂,₃ receptor subunits. Within the SVZ the majority of cells have an unexpected nuclear GABA(A)R β₂,₃ expression that is inversely proportional to that of PCNA (proliferating cell nuclear antigen marker), which is a very different pattern of expression compared with underlying caudate nucleus cells. Taken together our results provide a detailed description of the chemo-architecture of the adult human SVZ demonstrating the importance of GABA and GABA(A) receptors on the various cell types in the SVZ. (Copyright © 2013 Elsevier B.V. All rights reserved.) |
| فهرسة مساهمة: | Keywords: GABA; GABA(A) receptor; Human brain; Progenitor cell; Subventricular zone |
| المشرفين على المادة: | 0 (Protein Subunits) 0 (Receptors, GABA-A) |
| تواريخ الأحداث: | Date Created: 20130618 Date Completed: 20140502 Latest Revision: 20130916 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1016/j.jchemneu.2013.06.001 |
| PMID: | 23770130 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1873-6300 |
|---|---|
| DOI: | 10.1016/j.jchemneu.2013.06.001 |