دورية أكاديمية
Simple, direct drug susceptibility testing technique for diagnosis of drug-resistant tuberculosis in resource-poor settings.
| العنوان: | Simple, direct drug susceptibility testing technique for diagnosis of drug-resistant tuberculosis in resource-poor settings. |
|---|---|
| المؤلفون: | Kim CK; Korean National Tuberculosis Association, Korean Institute of Tuberculosis, Republic of Korea. psoas95@gmail.com, Joo YT, Lee EP, Park YK, Kim HJ, Kim SJ |
| المصدر: | The international journal of tuberculosis and lung disease : the official journal of the International Union against Tuberculosis and Lung Disease [Int J Tuberc Lung Dis] 2013 Sep; Vol. 17 (9), pp. 1212-6. Date of Electronic Publication: 2013 Jul 02. |
| نوع المنشور: | Comparative Study; Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: The Union Country of Publication: France NLM ID: 9706389 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1815-7920 (Electronic) Linking ISSN: 10273719 NLM ISO Abbreviation: Int J Tuberc Lung Dis Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Paris, France : The Union, c1997- |
| مواضيع طبية MeSH: | Antitubercular Agents*/economics , Antitubercular Agents*/therapeutic use , Drug Resistance, Multiple, Bacterial*, Developing Countries/*economics , Microbial Sensitivity Tests/*methods , Mycobacterium tuberculosis/*drug effects , Tuberculosis, Multidrug-Resistant/*diagnosis , Tuberculosis, Pulmonary/*diagnosis, Calibration ; Cost-Benefit Analysis ; Dose-Response Relationship, Drug ; Health Care Costs ; Health Resources/economics ; Humans ; Microbial Sensitivity Tests/economics ; Microbial Sensitivity Tests/standards ; Mycobacterium tuberculosis/growth & development ; Predictive Value of Tests ; Prognosis ; Reference Standards ; Sputum/microbiology ; Tuberculosis, Multidrug-Resistant/drug therapy ; Tuberculosis, Multidrug-Resistant/economics ; Tuberculosis, Multidrug-Resistant/microbiology ; Tuberculosis, Pulmonary/drug therapy ; Tuberculosis, Pulmonary/microbiology |
| مستخلص: | Setting: The Korean Institute of Tuberculosis, Seoul, Republic of Korea. Objective: To develop a simple, direct drug susceptibility testing (DST) technique using Kudoh-modified Ogawa (KMO) medium. Design: The critical concentrations of isoniazid (INH), rifampicin (RMP), kanamycin (KM) and ofloxacin (OFX) for KMO medium were calibrated by comparing the minimal inhibitory concentrations (MICs) against clinical isolates of Mycobacterium tuberculosis on KMO with those on Löwenstein-Jensen (LJ). The performance of the direct KMO DST technique was evaluated on 186 smear-positive sputum specimens and compared with indirect LJ DST. Results: Agreement of MICs on direct vs. indirect DST was high for INH, RMP and OFX. KM MICs on KMO were ∼10 g/ml higher than those on LJ. The critical concentrations of INH, RMP, OFX and KM for KMO were therefore set at 0.2, 40.0, 2.0, and 40.0 g/ml. The evaluation of direct DST of smear-positive sputum specimens showed 100% agreement with indirect LJ DST for INH and RMP. However, the respective susceptible and resistant predictive values were 98.8% and 100% for OFX, and 100% and 80% for KM. Conclusion: Direct DST using KMO is useful, with clear advantages of a shorter turnaround time, procedural simplicity and low cost compared to indirect DST. It may be most indicated in resource-poor settings for programmatic management of drug-resistant tuberculosis. |
| المشرفين على المادة: | 0 (Antitubercular Agents) |
| تواريخ الأحداث: | Date Created: 20130705 Date Completed: 20140228 Latest Revision: 20151119 |
| رمز التحديث: | 20231215 |
| DOI: | 10.5588/ijtld.12.1009 |
| PMID: | 23823178 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1815-7920 |
|---|---|
| DOI: | 10.5588/ijtld.12.1009 |