Differential diagnosis of reactive mesothelial cells and malignant mesothelioma cells using the cell proliferation markers minichromosome maintenance protein 7, geminin, topoisomerase II alpha and Ki-67.

التفاصيل البيبلوغرافية
العنوان:	Differential diagnosis of reactive mesothelial cells and malignant mesothelioma cells using the cell proliferation markers minichromosome maintenance protein 7, geminin, topoisomerase II alpha and Ki-67.
المؤلفون:	Kimura F; Department of Pathological Analysis, Division of Medical Life Sciences, Hirosaki University Graduate School of Health Sciences, Hirosaki, Japan. kimura.f.aa@m.titech.ac.jp, Okayasu I, Kakinuma H, Satoh Y, Kuwao S, Saegusa M, Watanabe J
المصدر:	Acta cytologica [Acta Cytol] 2013; Vol. 57 (4), pp. 384-90. Date of Electronic Publication: 2013 Jul 12.
نوع المنشور:	Journal Article
اللغة:	English
بيانات الدورية:	Publisher: Karger Country of Publication: Switzerland NLM ID: 0370307 Publication Model: Print-Electronic Cited Medium: Print ISSN: 0001-5547 (Print) Linking ISSN: 00015547 NLM ISO Abbreviation: Acta Cytol Subsets: MEDLINE
أسماء مطبوعة:	Publication: 2011- : Basel : Karger Original Publication: Chicago : International Academy of Cytology
مواضيع طبية MeSH:	Cell Proliferation* , Diagnosis, Differential, Biomarkers, Tumor/analysis , Epithelium/pathology , Mesothelioma/diagnosis, Aged ; Antigens, Neoplasm/analysis ; Antigens, Neoplasm/biosynthesis ; Ascitic Fluid/metabolism ; Ascitic Fluid/pathology ; Cell Cycle Proteins/analysis ; Cell Cycle Proteins/biosynthesis ; DNA Topoisomerases, Type II/analysis ; DNA Topoisomerases, Type II/biosynthesis ; DNA-Binding Proteins/analysis ; DNA-Binding Proteins/biosynthesis ; Epithelium/metabolism ; Female ; Geminin ; Humans ; Image Interpretation, Computer-Assisted ; Immunohistochemistry ; Ki-67 Antigen/analysis ; Ki-67 Antigen/biosynthesis ; Male ; Mesothelioma/metabolism ; Middle Aged ; Minichromosome Maintenance Complex Component 7 ; Nuclear Proteins/analysis ; Nuclear Proteins/biosynthesis ; Pleural Effusion/metabolism ; Pleural Effusion/pathology ; Sensitivity and Specificity
مستخلص:	Objective: The aim of this study was to evaluate whether the immunocytochemical expression of cell proliferation markers, such as minichromosome maintenance protein 7 (MCM 7), geminin, topoisomerase II alpha (topo IIα) and Ki-67, which are different types of cell proliferation markers, could be useful for their differential diagnosis in reactive mesothelial cells and malignant mesothelioma cells obtained from body cavity fluids. Study Design: Samples diagnosed and later histologically confirmed as reactive mesothelial cells (39 cases) or malignant mesothelioma (32 cases) in body cavity fluids were examined. Immunocytochemical staining of MCM 7, geminin, topo IIα and Ki-67 was performed with the immunoperoxidase polymer method. Results: Labeling indices (LIs) of MCM 7 (cutoff value 20.0%; sensitivity 100%; specificity 100%), geminin (cutoff value 4.5%; sensitivity 88.0%; specificity 70.0%), topo IIα (cutoff value 11.0%; sensitivity 88.0%; specificity 92.0%) and Ki-67 (cutoff value 15.3%; sensitivity 78.0%; specificity 79.0%) of malignant mesothelioma cells were significantly higher than those of reactive mesothelial cells. Conclusion: LIs of MCM 7, geminin and topo IIα can be reliable tools for the differential diagnosis of reactive mesothelial cells and malignant mesothelioma cells. (Copyright © 2013 S. Karger AG, Basel.)
المشرفين على المادة:	0 (Antigens, Neoplasm) 0 (Biomarkers, Tumor) 0 (Cell Cycle Proteins) 0 (DNA-Binding Proteins) 0 (GMNN protein, human) 0 (Geminin) 0 (Ki-67 Antigen) 0 (Nuclear Proteins) EC 3.6.4.12 (MCM7 protein, human) EC 3.6.4.12 (Minichromosome Maintenance Complex Component 7) EC 5.99.1.3 (DNA Topoisomerases, Type II)
تواريخ الأحداث:	Date Created: 20130718 Date Completed: 20131017 Latest Revision: 20220321
رمز التحديث:	20221213
DOI:	10.1159/000350262
PMID:	23860238
قاعدة البيانات:	MEDLINE

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تدمد:	0001-5547
DOI:	10.1159/000350262