دورية أكاديمية
Prediction and modulation of platelet recovery by discontinuous centrifugation of whole blood for the preparation of pure platelet-rich plasma.
العنوان: | Prediction and modulation of platelet recovery by discontinuous centrifugation of whole blood for the preparation of pure platelet-rich plasma. |
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المؤلفون: | Perez AG; Department of Materials and Bioprocesses Engineering, School of Chemical Engineering, University of Campinas , Campinas, Brazil ., Lichy R, Lana JF, Rodrigues AA, Luzo AC, Belangero WD, Santana MH |
المصدر: | BioResearch open access [Biores Open Access] 2013 Aug; Vol. 2 (4), pp. 307-14. |
نوع المنشور: | Journal Article |
اللغة: | English |
بيانات الدورية: | Publisher: Mary Ann Liebert Country of Publication: United States NLM ID: 101579333 Publication Model: Print Cited Medium: Print ISSN: 2164-7844 (Print) Linking ISSN: 21647844 NLM ISO Abbreviation: Biores Open Access Subsets: PubMed not MEDLINE |
أسماء مطبوعة: | Original Publication: New Rochelle, NY : Mary Ann Liebert |
مستخلص: | The aim of this study was to describe the behavior of the separation of red blood cells (RBCs) by discontinuous centrifugation (DC) of whole blood to modulate and control the platelet recovery in the preparation of pure platelet-rich plasma (P-PRP). P-PRP is a platelet-rich plasma (PRP) in which the white blood cell layer is not included. To achieve this goal, an analytical model was derived that takes into account the packing of RBCs and predicts the behavior of platelet and plasma recovery efficiencies (PtPlRE) based on the volume of whole blood, the hematocrit, and the volume of supernatant, as a function of the operating variables, centrifugal acceleration, and time. The model was derived from the basic equation of DC, which originates from the equilibrium balance of forces on a particle, and included the addition of one factor that corrected the terminal velocity of RBCs and was also correlated to the PtPlRE in the supernatant. This factor was the ratio between the fractional volume concentrations of plasma and RBCs in the centrifugation pellet after centrifugation. The model was validated and the variability of the data was determined using experimental data from 10 healthy donors in the age range of 25-35 years. The predicted behavior for the packing of RBCs and the PtPlRE was consistent with the behavior seen in the experimental data. Thus, the PtPlRE could be modulated and controlled through centrifugal acceleration, time, and hematocrit. Use of this model based on a physical description of events is the first step of a reliable standardization of PRP preparations. |
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فهرسة مساهمة: | Keywords: biomaterials; bioprocessing; regeneration; tissue engineering; wounds |
تواريخ الأحداث: | Date Created: 20130806 Date Completed: 20130805 Latest Revision: 20211021 |
رمز التحديث: | 20231215 |
مُعرف محوري في PubMed: | PMC3731689 |
DOI: | 10.1089/biores.2013.0015 |
PMID: | 23914337 |
قاعدة البيانات: | MEDLINE |
تدمد: | 2164-7844 |
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DOI: | 10.1089/biores.2013.0015 |