دورية أكاديمية
Morphometric evaluation of the spermatogonial stem cell distribution and niche in vertebrates.
| العنوان: | Morphometric evaluation of the spermatogonial stem cell distribution and niche in vertebrates. |
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| المؤلفون: | Campos-Junior PH; Laboratory of Cellular Biology, Department of Morphology, Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte, Brazil., Costa GM, de Avelar GF, Segatelli TM, Lacerda SM, Aponte PM, de França LR |
| المصدر: | Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2013; Vol. 1035, pp. 35-42. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Humana Press Country of Publication: United States NLM ID: 9214969 Publication Model: Print Cited Medium: Internet ISSN: 1940-6029 (Electronic) Linking ISSN: 10643745 NLM ISO Abbreviation: Methods Mol Biol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Totowa, NJ : Humana Press Original Publication: Clifton, N.J. : Humana Press, |
| مواضيع طبية MeSH: | Software* , Stem Cell Niche*, Adult Stem Cells/*metabolism, Animals ; Glial Cell Line-Derived Neurotrophic Factor Receptors/metabolism ; Horses ; Immunohistochemistry ; Male ; Mice ; Seminiferous Tubules/cytology ; Spermatogenesis |
| مستخلص: | Morphometry is a classical quantitative method often used in biology to provide a data basis for functional interpretations/interactions of a particular organ or system. Herein we took advantage of this valuable approach to evaluate the spermatogonial stem cell niche using the horse testis and immunocytochemical localization of GFRA1 [glial cell line-derived neurotrophic factor receptor produced by Sertoli cells)] as an example. Using the NIH ImageJ free software, we describe in detail all the necessary steps to investigate this specific and crucial microenvironment. Based on several recently published papers from our research group, this approach has proved to be fast, simple, and adaptable to a wide range of species and has the potential to be easily reproducible in different laboratories. |
| المشرفين على المادة: | 0 (Glial Cell Line-Derived Neurotrophic Factor Receptors) |
| تواريخ الأحداث: | Date Created: 20130821 Date Completed: 20140303 Latest Revision: 20130820 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1007/978-1-62703-508-8_4 |
| PMID: | 23959980 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1940-6029 |
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| DOI: | 10.1007/978-1-62703-508-8_4 |