دورية أكاديمية
أعرض في EDS

The role of caspase activation and mitochondrial depolarisation in cultured human apoptotic eosinophils.

التفاصيل البيبلوغرافية
العنوان: The role of caspase activation and mitochondrial depolarisation in cultured human apoptotic eosinophils.
المؤلفون: Alenzi FQ; Department of Med. Lab. Sci., College of Appl. Med. Sci., Al-Kharaj University, PO Box 422, Al-Kharj 11942, Saudi Arabia., Alenazi BQ, Al-Anazy FH, Mubaraki AM, Salem ML, Al-Jabri AA, Lotfy M, Bamaga MS, Alrabia MW, Wyse RK
المصدر: Saudi journal of biological sciences [Saudi J Biol Sci] 2010 Jan; Vol. 17 (1), pp. 29-36.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Saudi Biological Society Country of Publication: Saudi Arabia NLM ID: 101543796 Publication Model: Print Cited Medium: Print ISSN: 1319-562X (Print) Linking ISSN: 22137106 NLM ISO Abbreviation: Saudi J Biol Sci Subsets: PubMed not MEDLINE
أسماء مطبوعة: Original Publication: Riyadh : Saudi Biological Society : King Saud University, [2009]-
مستخلص: Caspases are key intracellular molecules in the control of apoptosis, but little is known concerning their relative contribution to the cascade of events leading to eosinophil apoptosis. We examined caspase-3, -8, and -9 activities in receptor ligation dependent apoptosis induction in the cultured eosinophils (CE). CE cultured alone for 48 hours exhibited constitutive apoptosis (12% ± 1.2). Significant (P < 0.05) enhancement of eosinophil apoptosis was observed following monoclonal antibody (Mab) treatment with CD45 (40% ± 0.7), CD95 (36% ± 1.6), or CD69 (34% ± 0.2). Caspase activity was analysed using the novel CaspaTagTM technique and flow cytometry. CE ligated with CD45 (Bra55), CD95 (Fas) and CD69 Mab resulted in caspase-3 and -9 activation after 16 hours post-ligation. This trend in caspase-3 and -9 activation continued to increase significantly through to the 20 and 24 hours time points when compared to isotype control. Activated up-stream caspase-8 was detected 16 and 20 hours after treatment with CD45, CD95 and CD69 Mab followed by a trend toward basal levels at 24 hours. Ligation of CD95 was followed by mitochondrial permeabilization, as demonstrated by marked increase in mitochondrial transmembrane potential ([Formula: see text]) at all time points. However, ligation with CD45 and CD69 failed to induce a change in [Formula: see text] at 16 hours post-treatment compared to isotype control even though there was an alteration in mitochondrial downstream-caspase activity following ligation with these Mab(s) at this time point. At 20 and 24 hours post-ligation, CD45 or CD69 induce significantly altered levels of [Formula: see text]. Thus, the intrinsic and extrinsic caspase pathways are involved in controlling receptor ligation-mediated apoptosis induction in human eosinophils, findings that may aid the development of a more targeted, anti inflammatory therapy for asthma.
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فهرسة مساهمة: Keywords: Apoptosis; Asthma; Caspases; Eosinophils; Mitochondria
تواريخ الأحداث: Date Created: 20130821 Date Completed: 20130820 Latest Revision: 20211021
رمز التحديث: 20240628
مُعرف محوري في PubMed: PMC3730707
DOI: 10.1016/j.sjbs.2009.12.005
PMID: 23961055
قاعدة البيانات: MEDLINE
الوصف
تدمد:1319-562X
DOI:10.1016/j.sjbs.2009.12.005