دورية أكاديمية
Derivation and culture of canine embryonic stem cells.
| العنوان: | Derivation and culture of canine embryonic stem cells. |
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| المؤلفون: | Tobias IC; Department of Physiology and Pharmacology, Schulich School of Medicine and Dentistry, University of Western Ontario, London, ON, Canada., Brooks CR, Teichroeb JH, Betts DH |
| المصدر: | Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2013; Vol. 1074, pp. 69-83. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Humana Press Country of Publication: United States NLM ID: 9214969 Publication Model: Print Cited Medium: Internet ISSN: 1940-6029 (Electronic) Linking ISSN: 10643745 NLM ISO Abbreviation: Methods Mol Biol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Totowa, NJ : Humana Press Original Publication: Clifton, N.J. : Humana Press, |
| مواضيع طبية MeSH: | Blastocyst/*cytology , Cell Culture Techniques/*methods , Embryonic Stem Cells/*cytology, Animals ; Cell Differentiation ; Cell Line ; Cryopreservation ; Dogs ; Embryonic Stem Cells/metabolism ; Humans ; Karyotyping |
| مستخلص: | The derivation of canine embryonic stem cells (cESCs) represents a significant achievement and opens the door to further stem cell research and therapies in the dog. Canines share a common environment with humans and exhibit a host of genetic diseases, many of which have human parallels. Thus, the canine model presents unique advantages over other currently used organisms to help develop stem cell therapies in humans. To reveal the therapeutic potential of cESCs further basic research on the molecular mechanisms controlling their pluripotency and self-renewal characteristics is needed. Herein, we present the methods for derivation and culture of cESCs. Following collection of the canine blastocyst, two derivation methods are presented; immunodissection and whole blastocyst explant. These two methods lead to cESCs differing in morphology and subculture techniques. Additional protocols for subculture of established lines, feeder-free culture, and cryopreservation protocols are also described. |
| تواريخ الأحداث: | Date Created: 20130827 Date Completed: 20140317 Latest Revision: 20130826 |
| رمز التحديث: | 20240829 |
| DOI: | 10.1007/978-1-62703-628-3_6 |
| PMID: | 23975806 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1940-6029 |
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| DOI: | 10.1007/978-1-62703-628-3_6 |