دورية أكاديمية
Reduction of carrageenan-induced acute pulmonary inflammation in mice by novel thiazolidinedione derivative LPSF/RA-4.
| العنوان: | Reduction of carrageenan-induced acute pulmonary inflammation in mice by novel thiazolidinedione derivative LPSF/RA-4. |
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| المؤلفون: | Barbosa KP; Laboratório de Ultraestrutura, Instituto Aggeu Magalhães, FIOCRUZ, Recife, Brazil. Electronic address: karlapsb@gmail.com., Santos LA, Ribeiro EL, Fragoso IT, Rocha SW, Nunes AK, França ME, Silva BS, Silva AK, Donato MA, Gomes FO, Silva TG, Pitta IR, Pitta MR, Lima MC, Uchôa FD, Galdino SL, Peixoto CA |
| المصدر: | European journal of pharmacology [Eur J Pharmacol] 2013 Oct 15; Vol. 718 (1-3), pp. 197-205. Date of Electronic Publication: 2013 Sep 12. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Science Country of Publication: Netherlands NLM ID: 1254354 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-0712 (Electronic) Linking ISSN: 00142999 NLM ISO Abbreviation: Eur J Pharmacol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2005- : Amsterdam : Elsevier Science Original Publication: Amsterdam, North Holland Pub. Co. |
| مواضيع طبية MeSH: | Carrageenan/*adverse effects , Indoles/*pharmacology , Pneumonia/*chemically induced , Pneumonia/*drug therapy , Thiazolidinediones/*pharmacology, Acute Disease ; Animals ; Gene Expression Regulation, Enzymologic/drug effects ; Indoles/therapeutic use ; Inflammation Mediators/metabolism ; Leukocyte Count ; Leukocytes, Mononuclear/cytology ; Leukocytes, Mononuclear/drug effects ; Leukocytes, Mononuclear/immunology ; Lung/drug effects ; Lung/pathology ; Male ; Mice ; Nitric Oxide/metabolism ; Pleurisy/chemically induced ; Pleurisy/drug therapy ; Pneumonia/blood ; Pneumonia/genetics ; Thiazolidinediones/therapeutic use |
| مستخلص: | A number of studies have demonstrated the biological activities of peroxisome proliferator-activated receptors. However, few studies have addressed the effects of the agonists of these receptors on lung diseases. The aim of the present study was to evaluate the anti-inflammatory action of a novel synthetic thiazolidine derivative (5Z)-3-benzyl-5-(1H-indol-3-ylmethylene)-thiazolidine-2,4-dione (LPSF/RA-4) on acute lung inflammation (pleurisy) induced by carrageenan. Forty mice were randomly allocated to the following groups: (I) saline control group (sham); (II) carrageenan (CAR) group; (III) CAR+LPSF/RA-4 group treated with LPSF/RA-4 (60 μmol/kg); and (IV) INDO group treated with indometacin (5mg/kg). Total cell counts and the measure of nitric oxide (NO) were performed in pleural exudates. Lung fragments were processed for light microscopy, transmission electron microscopy, immunohistochemistry and Western blotting. The influx of leucocytes and NO levels were significantly reduced following treatment with LPSF/RA-4 and INDO. Histopathological and ultrastructural analyses of the CAR group revealed evident tissue alterations, such as oedema, infiltrates of inflammatory cells and emphysema. These alterations were significantly reduced in the groups treated with LPSF/RA-4 or INDO. Immunohistochemistry revealed an increase in inflammatory markers (COX-2, iNOS, TNF-α and IL-1β) in the lung tissue of the CAR group, whereas the groups treated with LPSF/RA-4 and INDO exhibited significant reductions in such immunomarkers. Western blot analysis revealed an increased expression of COX-2 and IL-1 in the CAR group, which was reduced by treatment with LPSF/RA-4. The present findings demonstrate the potent anti-inflammatory action of the novel derivative thiazolidinedione LPSF/RA-4 in acute lung injury induced by carrageenan. (© 2013 Elsevier B.V. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Acute lung injury; Peroxisome proliferator-activated receptor; Pleurisy; Thiazolidinedione |
| المشرفين على المادة: | 0 (3-benzyl-5-(1H-indol-3-ylmethylene)-thiazolidine-2,4-dione) 0 (Indoles) 0 (Inflammation Mediators) 0 (Thiazolidinediones) 31C4KY9ESH (Nitric Oxide) 9000-07-1 (Carrageenan) AA68LXK93C (2,4-thiazolidinedione) |
| تواريخ الأحداث: | Date Created: 20130917 Date Completed: 20140610 Latest Revision: 20161125 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1016/j.ejphar.2013.08.033 |
| PMID: | 24036257 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1879-0712 |
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| DOI: | 10.1016/j.ejphar.2013.08.033 |