دورية أكاديمية
Generation of reactive oxygen species by leukocytes of Prochilodus lineatus.
| العنوان: | Generation of reactive oxygen species by leukocytes of Prochilodus lineatus. |
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| المؤلفون: | de Faria MT; Brazilian Agricultural Research Corporation, Embrapa, Trav. Dr. Enéas Pinheiro S/N, Caixa Postal 48, Belém, PA, 66095-130, Brazil, marcos.tucunduva@embrapa.br., Cury-Boaventura MF, Lopes LR, da Silva JR |
| المصدر: | Fish physiology and biochemistry [Fish Physiol Biochem] 2014 Apr; Vol. 40 (2), pp. 445-55. Date of Electronic Publication: 2013 Sep 26. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Kluwer Academic Publishers Country of Publication: Netherlands NLM ID: 100955049 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1573-5168 (Electronic) Linking ISSN: 09201742 NLM ISO Abbreviation: Fish Physiol Biochem Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Dordrecht ; Boston : Kluwer Academic Publishers Original Publication: Amsterdam ; Berkeley : Kugler, 1986- |
| مواضيع طبية MeSH: | Characiformes/*metabolism, Animals ; Brazil ; Characiformes/blood ; Characiformes/immunology ; Female ; Fish Proteins/metabolism ; Humans ; Immunity, Innate ; Kidney/metabolism ; Leukocytes/immunology ; Leukocytes/metabolism ; Leukocytes/ultrastructure ; Male ; Mitochondria/metabolism ; NADPH Oxidases/metabolism ; Reactive Oxygen Species/metabolism ; Respiratory Burst |
| مستخلص: | Prochilodus lineatus (curimbatá), from the Procholodontidae family, is a Brazilian freshwater fish, which is important commercially, nutritionally and ecologically. It is encountered in the Rio da Prata Bay in Southern South America. Studies on the immune system of this fish are scarce, but the physiological mechanisms of the species are analogous to those of other vertebrates. Thus, this work discusses the present study, which correlates P. lineatus leukocytes and the generation of reactive oxygen species after modulatory stimuli. Leukocytes were characterized by light and electron transmission microscopy and investigated by the generation of H2O2 and O2 (-), using phenol red, flow-cytometry and electron transmission histochemistry. The study determined that monocytes and neutrophils are the main cells responsible for generating O2 after stimulation with phorbol myristate acetate. Superoxide dismutase successfully inhibited the generation of reactive oxygen species in neutrophils and monocytes, but stimulated generation when in association with phorbol myristate acetate. Fish leukocyte samples from P. lineatus showed cross-reactivity with antibodies directed against human NADPH-oxidase antibody subunits (p47(phox) and p67(phox)). Thus, catalase enhanced the presence of p47(phox). Neutrophil mitochondria were shown to be generators of H2O2 (charged by cerium precipitate), being enlarged and changing their format. The present study contributes to a better understanding of the respiratory burst pathways in this species and suggests mitochondria as the organelle responsible for generation of reactive oxygen species. |
| References: | J Comp Pathol. 2001 Aug-Oct;125(2-3):174-81. (PMID: 11578134) Theriogenology. 2011 Mar 15;75(5):951-61. (PMID: 21247623) Parasitology. 2001;123 Suppl:S159-67. (PMID: 11769280) Fish Shellfish Immunol. 2006 Feb;20(2):239-62. (PMID: 15950490) Mar Environ Res. 2007 Aug;64(2):181-90. (PMID: 17316785) Int J Biochem Cell Biol. 2005 Dec;37(12):2478-503. (PMID: 16103002) Adv Enzyme Regul. 2004;44:279-98. (PMID: 15581496) Dev Comp Immunol. 1991 Fall;15(4):431-6. (PMID: 1723051) Aquat Toxicol. 2007 May 1;82(2):135-43. (PMID: 17374408) Dev Comp Immunol. 2001 Oct-Dec;25(8-9):807-25. (PMID: 11602197) Mol Immunol. 2008 Mar;45(6):1720-31. (PMID: 17997160) Nutrition. 2005 Mar;21(3):395-405. (PMID: 15797684) Hypertension. 2005 May;45(5):847-8. (PMID: 15837827) J Exp Biol. 2011 Nov 15;214(Pt 22):3732-41. (PMID: 22031737) Comp Biochem Physiol A Mol Integr Physiol. 2010 May;156(1):50-6. (PMID: 20060057) J Fish Dis. 2003 Sep;26(9):499-509. (PMID: 14575368) Dev Comp Immunol. 2004 Jul 1;28(9):911-25. (PMID: 15183032) J Immunol Methods. 1980;38(1-2):161-70. (PMID: 6778929) Exp Mol Med. 2009 Apr 30;41(4):217-25. (PMID: 19372727) J Cell Biol. 1975 Dec;67(3):566-86. (PMID: 407) Cardiovasc Res. 2005 Jan 1;65(1):16-27. (PMID: 15621030) J Immunol Methods. 1981;46(2):211-26. (PMID: 6273471) Dev Comp Immunol. 1996 May-Jun;20(3):207-16. (PMID: 8955595) Fish Shellfish Immunol. 2003 Jul;15(1):29-38. (PMID: 12787685) Ecotoxicol Environ Saf. 2007 Nov;68(3):372-8. (PMID: 17261332) Dev Comp Immunol. 2001 Jun-Jul;25(5-6):461-5. (PMID: 11356225) Front Biosci. 2007 Jan 01;12:1229-37. (PMID: 17127376) Dev Comp Immunol. 1996 Sep-Oct;20(5):323-30. (PMID: 9016386) Fish Shellfish Immunol. 2000 Nov;10(8):725-9. (PMID: 11185756) Comp Biochem Physiol C Toxicol Pharmacol. 2007 Mar;145(2):288-93. (PMID: 17300992) J Leukoc Biol. 1990 May;47(5):440-8. (PMID: 2159514) Biochem J. 2005 Mar 1;386(Pt 2):255-61. (PMID: 15491278) |
| المشرفين على المادة: | 0 (Fish Proteins) 0 (Reactive Oxygen Species) EC 1.6.3.- (NADPH Oxidases) |
| تواريخ الأحداث: | Date Created: 20130927 Date Completed: 20150219 Latest Revision: 20220317 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1007/s10695-013-9856-9 |
| PMID: | 24068363 |
| قاعدة البيانات: | MEDLINE |
Find this article in full text from Springer Verlag. 
Full Text Finder | تدمد: | 1573-5168 |
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| DOI: | 10.1007/s10695-013-9856-9 |