دورية أكاديمية
Generation of reactive oxygen species by leukocytes of Prochilodus lineatus.
العنوان: | Generation of reactive oxygen species by leukocytes of Prochilodus lineatus. |
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المؤلفون: | de Faria MT; Brazilian Agricultural Research Corporation, Embrapa, Trav. Dr. Enéas Pinheiro S/N, Caixa Postal 48, Belém, PA, 66095-130, Brazil, marcos.tucunduva@embrapa.br., Cury-Boaventura MF, Lopes LR, da Silva JR |
المصدر: | Fish physiology and biochemistry [Fish Physiol Biochem] 2014 Apr; Vol. 40 (2), pp. 445-55. Date of Electronic Publication: 2013 Sep 26. |
نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
اللغة: | English |
بيانات الدورية: | Publisher: Kluwer Academic Publishers Country of Publication: Netherlands NLM ID: 100955049 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1573-5168 (Electronic) Linking ISSN: 09201742 NLM ISO Abbreviation: Fish Physiol Biochem Subsets: MEDLINE |
أسماء مطبوعة: | Publication: Dordrecht ; Boston : Kluwer Academic Publishers Original Publication: Amsterdam ; Berkeley : Kugler, 1986- |
مواضيع طبية MeSH: | Characiformes/*metabolism, Animals ; Brazil ; Characiformes/blood ; Characiformes/immunology ; Female ; Fish Proteins/metabolism ; Humans ; Immunity, Innate ; Kidney/metabolism ; Leukocytes/immunology ; Leukocytes/metabolism ; Leukocytes/ultrastructure ; Male ; Mitochondria/metabolism ; NADPH Oxidases/metabolism ; Reactive Oxygen Species/metabolism ; Respiratory Burst |
مستخلص: | Prochilodus lineatus (curimbatá), from the Procholodontidae family, is a Brazilian freshwater fish, which is important commercially, nutritionally and ecologically. It is encountered in the Rio da Prata Bay in Southern South America. Studies on the immune system of this fish are scarce, but the physiological mechanisms of the species are analogous to those of other vertebrates. Thus, this work discusses the present study, which correlates P. lineatus leukocytes and the generation of reactive oxygen species after modulatory stimuli. Leukocytes were characterized by light and electron transmission microscopy and investigated by the generation of H2O2 and O2 (-), using phenol red, flow-cytometry and electron transmission histochemistry. The study determined that monocytes and neutrophils are the main cells responsible for generating O2 after stimulation with phorbol myristate acetate. Superoxide dismutase successfully inhibited the generation of reactive oxygen species in neutrophils and monocytes, but stimulated generation when in association with phorbol myristate acetate. Fish leukocyte samples from P. lineatus showed cross-reactivity with antibodies directed against human NADPH-oxidase antibody subunits (p47(phox) and p67(phox)). Thus, catalase enhanced the presence of p47(phox). Neutrophil mitochondria were shown to be generators of H2O2 (charged by cerium precipitate), being enlarged and changing their format. The present study contributes to a better understanding of the respiratory burst pathways in this species and suggests mitochondria as the organelle responsible for generation of reactive oxygen species. |
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المشرفين على المادة: | 0 (Fish Proteins) 0 (Reactive Oxygen Species) EC 1.6.3.- (NADPH Oxidases) |
تواريخ الأحداث: | Date Created: 20130927 Date Completed: 20150219 Latest Revision: 20220317 |
رمز التحديث: | 20221213 |
DOI: | 10.1007/s10695-013-9856-9 |
PMID: | 24068363 |
قاعدة البيانات: | MEDLINE |
تدمد: | 1573-5168 |
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DOI: | 10.1007/s10695-013-9856-9 |