دورية أكاديمية
Detection of contaminants in cell cultures, sera and trypsin.
| العنوان: | Detection of contaminants in cell cultures, sera and trypsin. |
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| المؤلفون: | Pinheiro de Oliveira TF; Laboratório de Biologia Molecular/Laboratório de Diagnóstico de Doenças Virais, Laboratório Nacional Agropecuário de Minas Gerais, Pedro Leopoldo, Minas Gerais, Brazil. Electronic address: oliveiratfp@yahoo.com.br., Fonseca AA Jr, Camargos MF, de Oliveira AM, Pinto Cottorello AC, Souza Ados R, de Almeida IG, Heinemann MB |
| المصدر: | Biologicals : journal of the International Association of Biological Standardization [Biologicals] 2013 Nov; Vol. 41 (6), pp. 407-14. Date of Electronic Publication: 2013 Sep 23. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Academic Press Country of Publication: England NLM ID: 9004494 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1095-8320 (Electronic) Linking ISSN: 10451056 NLM ISO Abbreviation: Biologicals Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: London ; San Diego : Academic Press, c1990- |
| مواضيع طبية MeSH: | Serum/*microbiology , Serum/*virology , Trypsin/*analysis, Animals ; Cattle ; Cell Line ; Cells, Cultured ; Circovirus/genetics ; DNA, Bacterial/genetics ; DNA, Bacterial/isolation & purification ; DNA, Viral/genetics ; DNA, Viral/isolation & purification ; Diarrhea Virus 1, Bovine Viral/genetics ; Drug Contamination ; Humans ; Laboratories/standards ; Laboratories/statistics & numerical data ; Leukemia Virus, Bovine/genetics ; Mycoplasma/genetics ; Mycoplasma Infections/blood ; Mycoplasma Infections/microbiology ; Polymerase Chain Reaction/methods ; RNA, Viral/genetics ; RNA, Viral/isolation & purification ; Reproducibility of Results ; Swine ; Virus Diseases/blood ; Virus Diseases/virology |
| مستخلص: | The aim of this study was standardization and application of polymerase chain reaction (PCR) for the detection of contaminants in cell cultures, sera and trypsin. Five PCR protocols were standardized to assess the presence of genetic material from mycoplasma, porcine circovirus 1 (PCV1), bovine leukemia virus (BLV) or bovine viral diarrhea virus (BVDV) in cell culture samples. PCR reactions for the genes GAPDH and beta-actin were used to evaluate the efficiency of nucleic acid extraction. The PCR protocols were applied to 88 cell culture samples from eight laboratories. The tests were also used to assess potential contamination in 10 trypsin samples and 13 fetal calf serum samples from different lots from five of the laboratories. The results showed the occurrence of the following as DNA cell culture contaminants: 34.1% for mycoplasma, 35.2% for PCV1, 23.9% for BVDV RNA and 2.3% for BLV. In fetal calf sera and trypsin samples BVDV RNA and PCV1 DNA was detected. The results demonstrated that cell culture, sera and trypsin used by different laboratories show a high rate of contaminants. The results highlight the need for monitoring cell cultures and controlling for biological contaminants in laboratories and cell banks working with these materials. (Copyright © 2013 The International Alliance for Biological Standardization. Published by Elsevier Ltd. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Cell cultures; Mollicutes; PCR; Sera; Trypsin; Viral contaminants |
| المشرفين على المادة: | 0 (DNA, Bacterial) 0 (DNA, Viral) 0 (RNA, Viral) EC 3.4.21.4 (Trypsin) |
| تواريخ الأحداث: | Date Created: 20130928 Date Completed: 20140725 Latest Revision: 20131122 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1016/j.biologicals.2013.08.005 |
| PMID: | 24071554 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1095-8320 |
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| DOI: | 10.1016/j.biologicals.2013.08.005 |