دورية أكاديمية
Intracellular cytokine and cathelicidin secretion from monocytes and neutrophils in childhood tuberculosis.
| العنوان: | Intracellular cytokine and cathelicidin secretion from monocytes and neutrophils in childhood tuberculosis. |
|---|---|
| المؤلفون: | Torun E; From the *Department of Pediatrics, Beazmialem Vakif University; †Department of Pediatric Pulmonology; and ‡Department of Immunology, Institute of Experimental Medicine (DETAE), Istanbul University, Istanbul, Turkey., Cakir E, Aktas EC, Gedik AH, Deniz G |
| المصدر: | The Pediatric infectious disease journal [Pediatr Infect Dis J] 2014 Feb; Vol. 33 (2), pp. 224-6. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Williams & Wilkins Country of Publication: United States NLM ID: 8701858 Publication Model: Print Cited Medium: Internet ISSN: 1532-0987 (Electronic) Linking ISSN: 08913668 NLM ISO Abbreviation: Pediatr Infect Dis J Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: [Baltimore, Md. : Williams & Wilkins, c1987- |
| مواضيع طبية MeSH: | Antimicrobial Cationic Peptides/*blood , Antimicrobial Cationic Peptides/*metabolism , Cytokines/*blood , Monocytes/*metabolism , Neutrophils/*metabolism , Tuberculosis, Pulmonary/*blood, Adolescent ; Antimicrobial Cationic Peptides/immunology ; Case-Control Studies ; Cells, Cultured ; Child ; Child, Preschool ; Cytokines/immunology ; Female ; Humans ; Infant ; Male ; Monocytes/immunology ; Neutrophils/immunology ; Statistics, Nonparametric ; Tuberculosis, Pulmonary/immunology ; Cathelicidins |
| مستخلص: | Human cathelicidin (hCAP 18) is one of the main immunomodulators in the local immune response to Mycobacterium tuberculosis. There has been no information regarding the role of hCAP 18+ in the in vivo production of cytokine in childhood pulmonary tuberculosis (TB). We aimed to determine the intracellular cytokine secretion, including hCAP 18+ from monocytes and neutrophils of pulmonary TB in children, compared with healthy children. Fifteen patients with pulmonary TB were enrolled in the study as the study group, and 15 healthy children as the control group, between the age of 1 and 16 years. The patients' 25 hydroxyvitamin D levels were measured. The expression of hCAP 18+, TNF α, INF-γ and IL-8 from CD14+ monocytes and CD15 + neutrophils was analyzed using the flow cytometry method. The statistical analysis was performed with PASW Statistics v.13.0. The mean vitamin D level was similar in both groups (P = 0.78). The expression of hCAP 18+ (P = 0.0001) and IL-8 from CD14 + (P = 0.0001) monocytes were significantly higher in the study group compared with the control group. There was no difference in the hCAP 18+ and IL-8 expression in CD 15+ neutrophils in both groups. The expression of TNF α, INF-γ from CD14+ monocytes and CD 15+ neutrophils, in both study and control groups revealed no statistical differences.The level of hCAP 18+ and IL-8 released from monocytes were enhanced in the serum in childhood pulmonary TB. The present study is the first report detecting the intracellular hCAP 18+ expression in vivo in childhood pulmonary TB. |
| المشرفين على المادة: | 0 (Antimicrobial Cationic Peptides) 0 (Cytokines) 0 (Cathelicidins) |
| تواريخ الأحداث: | Date Created: 20131010 Date Completed: 20140925 Latest Revision: 20221207 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1097/INF.0000000000000109 |
| PMID: | 24104956 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1532-0987 |
|---|---|
| DOI: | 10.1097/INF.0000000000000109 |