دورية أكاديمية
In vitro and in vivo effects of human monocytes and their subsets on new vessel formation.
| العنوان: | In vitro and in vivo effects of human monocytes and their subsets on new vessel formation. |
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| المؤلفون: | Czepluch FS; Department of Cardiology and Pulmonary Medicine, University Medical Center Göttingen, Göttingen, Germany., Bernhardt M, Kuschicke H, Gogiraju R, Schroeter MR, Riggert J, Hasenfuss G, Schäfer K |
| المصدر: | Microcirculation (New York, N.Y. : 1994) [Microcirculation] 2014 Feb; Vol. 21 (2), pp. 148-58. |
| نوع المنشور: | Clinical Trial; Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Wiley-Blackwell Country of Publication: United States NLM ID: 9434935 Publication Model: Print Cited Medium: Internet ISSN: 1549-8719 (Electronic) Linking ISSN: 10739688 NLM ISO Abbreviation: Microcirculation Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Malden, MA : Wiley-Blackwell Original Publication: New York, NY : Chapman & Hall, c1994- |
| مواضيع طبية MeSH: | Neovascularization, Physiologic*, Human Umbilical Vein Endothelial Cells/*metabolism , Monocytes/*metabolism, Adult ; Animals ; Cells, Cultured ; Heterografts ; Hindlimb/blood supply ; Human Umbilical Vein Endothelial Cells/cytology ; Humans ; Ischemia/physiopathology ; Ischemia/therapy ; Male ; Mice ; Mice, Nude ; Monocytes/cytology ; Monocytes/transplantation |
| مستخلص: | Objective: Human monocytes can be divided into CD16(-) monocytes and CD16(+) monocytes. Studies in mice suggested differential effects of monocyte subsets during new vessel formation. Methods: The functional role of human monocyte subsets in neovascularization processes was investigated. For in vivo experiments, nude mice underwent unilateral hindlimb ischemia surgery before being injected with either total monocytes, CD16(-) monocytes or CD16(+) monocytes isolated from healthy individuals. Results: In vitro, cytokine array analysis demonstrated that monocytes release numerous angiogenic cytokines, some of which were differentially expressed in monocyte subsets. Sprout length was enhanced in EC spheroids being cultured in conditioned medium obtained from total monocytes and, to a lesser extent, also in supernatants of CD16(-) monocytes. Laser Doppler perfusion imaging up to day 28 after surgery revealed a trend toward improved revascularization in mice treated with monocytes, but no significant differences between monocyte subsets. Histological analyses four weeks after surgery showed an increased arteriole size in mice having received CD16(+) monocytes, whereas the number of capillaries did not significantly differ between groups. Conclusions: Our findings suggest additive and differential effects of monocyte subsets during neovascularization processes, possibly due to an altered secretion of angiogenic factors and their paracrine capacity to stimulate new vessel formation. (© 2013 John Wiley & Sons Ltd.) |
| فهرسة مساهمة: | Keywords: CD14; CD16; angiogenesis; arteriogenesis; blood vessel; hindlimb ischemia; monocytes |
| تواريخ الأحداث: | Date Created: 20131016 Date Completed: 20151030 Latest Revision: 20140807 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1111/micc.12100 |
| PMID: | 24125396 |
| قاعدة البيانات: | MEDLINE |
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Full Text Finder | تدمد: | 1549-8719 |
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| DOI: | 10.1111/micc.12100 |