دورية أكاديمية
Compartmentalization of distinct cAMP signaling pathways in mammalian sperm.
| العنوان: | Compartmentalization of distinct cAMP signaling pathways in mammalian sperm. |
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| المؤلفون: | Wertheimer E; From the Department of Veterinary and Animal Sciences, University of Massachusetts, Amherst, Massachusetts 01003., Krapf D, de la Vega-Beltran JL, Sánchez-Cárdenas C, Navarrete F, Haddad D, Escoffier J, Salicioni AM, Levin LR, Buck J, Mager J, Darszon A, Visconti PE |
| المصدر: | The Journal of biological chemistry [J Biol Chem] 2013 Dec 06; Vol. 288 (49), pp. 35307-20. Date of Electronic Publication: 2013 Oct 15. |
| نوع المنشور: | Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Inc. on behalf of American Society for Biochemistry and Molecular Biology Country of Publication: United States NLM ID: 2985121R Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1083-351X (Electronic) Linking ISSN: 00219258 NLM ISO Abbreviation: J Biol Chem Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2021- : [New York, NY] : Elsevier Inc. on behalf of American Society for Biochemistry and Molecular Biology Original Publication: Baltimore, MD : American Society for Biochemistry and Molecular Biology |
| مواضيع طبية MeSH: | Adenylyl Cyclases/*metabolism , Cyclic AMP/*metabolism , Spermatozoa/*metabolism, Acrosome/metabolism ; Acrosome Reaction/drug effects ; Adenine/analogs & derivatives ; Adenine/pharmacology ; Adenylyl Cyclases/deficiency ; Adenylyl Cyclases/genetics ; Animals ; Calcium/metabolism ; Cell Compartmentation ; Colforsin/pharmacology ; Cyclic AMP-Dependent Protein Kinase Catalytic Subunits/metabolism ; Enzyme Inhibitors/pharmacology ; Female ; GTP-Binding Protein alpha Subunits, Gs/metabolism ; Male ; Mice ; Mice, Knockout ; Signal Transduction/drug effects ; Sperm Capacitation/drug effects ; Sperm Head/metabolism ; Sperm Tail/metabolism ; Spermatozoa/drug effects |
| مستخلص: | Fertilization competence is acquired in the female tract in a process known as capacitation. Capacitation is needed for the activation of motility (e.g. hyperactivation) and to prepare the sperm for an exocytotic process known as acrosome reaction. Although the HCO3(-)-dependent soluble adenylyl cyclase Adcy10 plays a role in motility, less is known about the source of cAMP in the sperm head. Transmembrane adenylyl cyclases (tmACs) are another possible source of cAMP. These enzymes are regulated by stimulatory heterotrimeric Gs proteins; however, the presence of Gs or tmACs in mammalian sperm has been controversial. In this study, we used Western blotting and cholera toxin-dependent ADP-ribosylation to show the Gs presence in the sperm head. Also, we showed that forskolin, a tmAC-specific activator, induces cAMP accumulation in sperm from both WT and Adcy10-null mice. This increase is blocked by the tmAC inhibitor SQ22536 but not by the Adcy10 inhibitor KH7. Although Gs immunoreactivity and tmAC activity are detected in the sperm head, PKA is only found in the tail, where Adcy10 was previously shown to reside. Consistent with an acrosomal localization, Gs reactivity is lost in acrosome-reacted sperm, and forskolin is able to increase intracellular Ca(2+) and induce the acrosome reaction. Altogether, these data suggest that cAMP pathways are compartmentalized in sperm, with Gs and tmAC in the head and Adcy10 and PKA in the flagellum. |
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| معلومات مُعتمدة: | R01 HD044044 United States HD NICHD NIH HHS; HD38082 United States HD NICHD NIH HHS; HD44044 United States HD NICHD NIH HHS; R01 GM062328 United States GM NIGMS NIH HHS; R01 HD038082 United States HD NICHD NIH HHS; GM62328 United States GM NIGMS NIH HHS; R01 HD059913 United States HD NICHD NIH HHS; HD059913 United States HD NICHD NIH HHS |
| فهرسة مساهمة: | Keywords: Acrosome Reaction; Adenylate Cyclase (Adenylyl Cyclase); Calcium Imaging; Cell Signaling; Cyclic AMP (cAMP); Forskolin; Heterotrimeric G Proteins; Protein Kinase A (PKA); Signal Transduction; Sperm Capacitation |
| المشرفين على المادة: | 0 (Enzyme Inhibitors) 17318-31-9 (9-(tetrahydro-2-furyl)-adenine) 1F7A44V6OU (Colforsin) E0399OZS9N (Cyclic AMP) EC 2.7.11.11 (Cyclic AMP-Dependent Protein Kinase Catalytic Subunits) EC 3.6.5.1 (GTP-Binding Protein alpha Subunits, Gs) EC 4.6.1.1 (Adenylyl Cyclases) JAC85A2161 (Adenine) SY7Q814VUP (Calcium) |
| تواريخ الأحداث: | Date Created: 20131017 Date Completed: 20140213 Latest Revision: 20211021 |
| رمز التحديث: | 20221213 |
| مُعرف محوري في PubMed: | PMC3853279 |
| DOI: | 10.1074/jbc.M113.489476 |
| PMID: | 24129574 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1083-351X |
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| DOI: | 10.1074/jbc.M113.489476 |