دورية أكاديمية
'Prevalence and antimicrobial susceptibility of Listeria monocytogenes and methicillin-resistant Staphylococcus aureus strains from raw meat and meat products in Zaria, Nigeria.
| العنوان: | 'Prevalence and antimicrobial susceptibility of Listeria monocytogenes and methicillin-resistant Staphylococcus aureus strains from raw meat and meat products in Zaria, Nigeria. |
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| المؤلفون: | Ndahi MD; Federal Department of Livestock and Pest Control Services, Jos, Plateau state, Nigeria., Kwaga JK, Bello M, Kabir J, Umoh VJ, Yakubu SE, Nok AJ |
| المصدر: | Letters in applied microbiology [Lett Appl Microbiol] 2014 Mar; Vol. 58 (3), pp. 262-9. Date of Electronic Publication: 2013 Nov 14. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Oxford University Press Country of Publication: England NLM ID: 8510094 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1472-765X (Electronic) Linking ISSN: 02668254 NLM ISO Abbreviation: Lett Appl Microbiol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2023- : Oxford : Oxford University Press Original Publication: Oxford, UK : Published for the Society for Applied Bacteriology by Blackwell Scientific Publications, [c1985- |
| مواضيع طبية MeSH: | Drug Resistance, Bacterial*, Listeria monocytogenes/*drug effects , Listeria monocytogenes/*isolation & purification , Meat/*microbiology , Methicillin-Resistant Staphylococcus aureus/*drug effects , Methicillin-Resistant Staphylococcus aureus/*isolation & purification, Animals ; Anti-Bacterial Agents/pharmacology ; Consumer Product Safety ; Food Contamination/analysis ; Food Contamination/statistics & numerical data ; Listeria/classification ; Listeria/drug effects ; Listeria/genetics ; Listeria/isolation & purification ; Listeria monocytogenes/genetics ; Meat Products/microbiology ; Methicillin-Resistant Staphylococcus aureus/genetics ; Microbial Sensitivity Tests ; Nigeria ; Staphylococcus/classification ; Staphylococcus/drug effects ; Staphylococcus/genetics ; Staphylococcus/isolation & purification |
| مستخلص: | Unlabelled: The bacterial genera Listeria and Staphylococcus have been frequently isolated from food products and are responsible for a number of animal and human diseases. The aim of the study was to simultaneously isolate and characterize L. monocytogenes and Staphylococcus species from 300 samples of raw meat and meat products, to determine the susceptibility of the organisms to commonly used antimicrobial agents and to determine the presence of haemolysin A (hyl) virulence gene in L. monocytogenes and staphylococcal cassette chromosome mecA (SCCmec) gene in the Staph. aureus isolates using PCR. Of the 85 Listeria isolates tested, 12 L. monocytogenes were identified and tested for their sensitivity to 14 antimicrobial agents. All the 12 isolates (100%) were resistant to nine antimicrobial agents, but however sensitive to gentamicin. Only one isolate was found to harbour the hylA gene. Twenty-nine isolates were confirmed as Staph. aureus by the Microbact 12S identification system and were all presumptively identified as methicillin-resistant Staph. aureus species using oxacillin-resistant Staph. aureus basal medium (ORSAB). The 29 Staph. aureus isolates were tested for their sensitivity to 16 antimicrobial agents, and 11 were resistant to methicillin. None of the 11 Staph. aureus isolates harboured the methicillin resistance, mecA gene. Significance and Impact of the Study: Listeria monocytogenes and Staphylococcus aureus are important agents of foodborne diseases. Occurrence of these infectious agents was established in meat and meat products in Zaria, Nigeria. Majority of isolates obtained from this study, displayed multidrug resistance to commonly used antimicrobial agents, including methicillin resistance among the Staph. aureus isolates. The potential virulence of L. monocytogenes found in ready-to-eat food was documented by the carriage of hly A gene by one of the isolates. A different mechanism of methicillin resistance or different homologue of mec A gene may be circulating among Nigerian isolates. (© 2013 The Society for Applied Microbiology.) |
| فهرسة مساهمة: | Keywords: Listeria monocytogenes; Staphylococcus aureus; antibiotic resistance; hlyA gene; meat products; mecA gene |
| المشرفين على المادة: | 0 (Anti-Bacterial Agents) |
| تواريخ الأحداث: | Date Created: 20131025 Date Completed: 20140613 Latest Revision: 20181202 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1111/lam.12183 |
| PMID: | 24151971 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1472-765X |
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| DOI: | 10.1111/lam.12183 |