دورية أكاديمية
أعرض في EDS

Nanocomposite-siRNA approach for down-regulation of VEGF and its receptor in myeloid leukemia cells.

التفاصيل البيبلوغرافية
العنوان: Nanocomposite-siRNA approach for down-regulation of VEGF and its receptor in myeloid leukemia cells.
المؤلفون: Wang L; Department of Hematology, Shanghai Ninth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, No 639 Zhizaoju Road, Shanghai, 200011, China., Zhang WJ; Department of Hematology, Tongji Hospital of Tongji University, No 389 Xincun Road, Shanghai, 200065, China. Electronic address: zhengwenjun@tongji.edu.cn., Xiu B; Department of Hematology, Tongji Hospital of Tongji University, No 389 Xincun Road, Shanghai, 200065, China. Electronic address: xiubing1233@tongji.edu.cn., Ding Y; Department of Hematology, Tongji Hospital of Tongji University, No 389 Xincun Road, Shanghai, 200065, China. Electronic address: topsnake2001@tongji.edu.cn., Li P; Department of Hematology, Tongji Hospital of Tongji University, No 389 Xincun Road, Shanghai, 200065, China. Electronic address: lilyforever76@hotmail.com., Ye WD; Department of Hematology, Shanghai Ninth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, No 639 Zhizaoju Road, Shanghai, 200011, China. Electronic address: ipcat@hotmail.com., Zhu Q; Department of Hematology, Shanghai Ninth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, No 639 Zhizaoju Road, Shanghai, 200011, China. Electronic address: zhuqiBM@163.com., Liang AB; Department of Hematology, Tongji Hospital of Tongji University, No 389 Xincun Road, Shanghai, 200065, China. Electronic address: lab7182@tongji.edu.cn.
المصدر: International journal of biological macromolecules [Int J Biol Macromol] 2014 Feb; Vol. 63, pp. 49-55. Date of Electronic Publication: 2013 Oct 29.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Elsevier Country of Publication: Netherlands NLM ID: 7909578 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-0003 (Electronic) Linking ISSN: 01418130 NLM ISO Abbreviation: Int J Biol Macromol Subsets: MEDLINE
أسماء مطبوعة: Publication: Amsterdam : Elsevier
Original Publication: Guildford, Eng., IPC Science and Technology Press.
مواضيع طبية MeSH: Chitosan/*chemistry , Leukemia, Myeloid/*genetics , Nanoparticles/*administration & dosage , RNA, Small Interfering/*administration & dosage , Vascular Endothelial Growth Factor A/*genetics, Cell Line, Tumor ; Cell Proliferation/drug effects ; Chitosan/administration & dosage ; Gene Expression Regulation, Neoplastic/drug effects ; Humans ; Leukemia, Myeloid/pathology ; Nanoparticles/chemistry ; Neovascularization, Pathologic/genetics ; Neovascularization, Pathologic/pathology ; RNA, Small Interfering/chemistry ; Receptors, Vascular Endothelial Growth Factor/genetics
مستخلص: Background: Efficient modulation of aberrant vascular endothelial growth factor (VEGF) and its receptor-1 (Flt-1) expressions have become a potential therapeutic strategy for hematologic malignancies including myeloid leukemia. In this study, we explored the safety and efficacy of chitosan nanoparticle siRNA-VEGF and Flt-1 in leukemic U973 cells.
Methods: Cell transfection efficiencies were analyzed by fluorescence microscope, quantitative Real Time PCR; cell growth inhibitory rates were analyzed by CCK-8 assays and flow cytometry.
Results: siRNA-coated chitosan nanosphere transfection led to 65%, Lipofectamine 2000 to 50% and adenovirus to 90% transfection efficiencies. Three days after transfection of U973 cells, the siRNA induced gene silencing rates of VEGF and Flt-1 were 68% and 65% in the adenovirus, 45% and 43% in the chitosan nanoparticle group. The cell growth inhibitory rates were 34.73% for VEGF and 27.61% for Flt-1 silencing in the adenovirus and 27.04% for VEGF and 21.49% for Flt-1 silencing in the chitosan nanoparticle group.
Conclusion: Chitosan nanoparticle siRNA technology can effectively inhibit the expression of VEGF and its receptor in leukemic cells, which led to suppression of their proliferation. Though less efficient than adenoviruses, their non-viral properties suggest that chitosan nanoparticle siRNA complex gene silencing is suitable for further trials.
(Copyright © 2013 Elsevier B.V. All rights reserved.)
فهرسة مساهمة: Keywords: Chitosan nanoparticles; RNA interference; Vascular endothelial growth factor (VEGF)
المشرفين على المادة: 0 (RNA, Small Interfering)
0 (VEGFA protein, human)
0 (Vascular Endothelial Growth Factor A)
9012-76-4 (Chitosan)
EC 2.7.10.1 (Receptors, Vascular Endothelial Growth Factor)
تواريخ الأحداث: Date Created: 20131105 Date Completed: 20140818 Latest Revision: 20131231
رمز التحديث: 20221213
DOI: 10.1016/j.ijbiomac.2013.10.028
PMID: 24183807
قاعدة البيانات: MEDLINE
الوصف
تدمد:1879-0003
DOI:10.1016/j.ijbiomac.2013.10.028