دورية أكاديمية
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Polyxylitol-based gene carrier improves the efficiency of gene transfer through enhanced endosomal osmolysis.

التفاصيل البيبلوغرافية
العنوان: Polyxylitol-based gene carrier improves the efficiency of gene transfer through enhanced endosomal osmolysis.
المؤلفون: Lee WS; Department of Agricultural Biotechnology, Seoul National University, Seoul, South Korea., Kim YK; Department of Pharmaceutics, School of Pharmacy, China Pharmaceutical University, Nanjing, China., Zhang Q; Department of Agricultural Biotechnology, Seoul National University, Seoul, South Korea., Park TE; Department of Agricultural Biotechnology, Seoul National University, Seoul, South Korea., Kang SK; Department of Agricultural Biotechnology, Seoul National University, Seoul, South Korea., Kim DW; National Institute of Animal Science, Rural Development Administration, Suwon, South Korea., Cho CS; Department of Agricultural Biotechnology, Seoul National University, Seoul, South Korea; Research Institute for Agriculture and Life Sciences, Seoul National University, Seoul, South Korea. Electronic address: chocs@plaza.snu.ac.kr., Choi YJ; Department of Agricultural Biotechnology, Seoul National University, Seoul, South Korea; Research Institute for Agriculture and Life Sciences, Seoul National University, Seoul, South Korea. Electronic address: cyjcow@snu.ac.kr.
المصدر: Nanomedicine : nanotechnology, biology, and medicine [Nanomedicine] 2014 Apr; Vol. 10 (3), pp. 525-34. Date of Electronic Publication: 2013 Oct 30.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Elsevier Country of Publication: United States NLM ID: 101233142 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1549-9642 (Electronic) Linking ISSN: 15499634 NLM ISO Abbreviation: Nanomedicine Subsets: MEDLINE
أسماء مطبوعة: Original Publication: New York, NY : Elsevier, c2005-
مواضيع طبية MeSH: DNA/*administration & dosage , Drug Carriers/*metabolism , Endosomes/*metabolism , Plasmids/*administration & dosage , Polyethyleneimine/*metabolism , Xylitol/*metabolism, Animals ; Cell Line ; DNA/genetics ; Drug Carriers/chemistry ; Humans ; Mice ; Osmotic Pressure ; Plasmids/genetics ; Polyethyleneimine/chemistry ; Polymers/chemistry ; Polymers/metabolism ; Transfection ; Xylitol/chemistry
مستخلص: Endosomal escape is one of the important processes for efficient non-viral gene delivery. In this study, we synthesized a novel non-viral vector called polyxylitol-based gene carrier (XGC) through a Miachael addition reaction between xylitol diacrylate as a crosslinking agent and low molecular weight polyethylenimine (PEI 1.2kDa). The small amount of xylitol integrated into XGC (3.9% w/w) contributed 50% of the osmotic pressure of XGC, and enhaned the osmolysis of endosome cooperatively with the proton sponge effect, thus improving endosomal escape. Furthermore, XGC showed higher transfection efficiency in vivo in muscle tissue than pDNA alone or PEI 25kDa. In conclusion, our results show that XGC enhanced transfection efficiency compared with PEI 25kDa, the golden standard non-viral gene carrier, by enhancing endosomal escape without increasing the number of transfected cells.
From the Clinical Editor: Enhanced gene delivery methods would greatly facilitate the development of gene therapies. These authors demonstrate that a polyxylitol-based gene carrier enhanced the transfection efficiency compared with the gold standard non-viral gene carrier, as a result of enhancing endosomal escape without increasing the number of transfected cells, warranting further studies of this method.
(Copyright © 2014 Elsevier Inc. All rights reserved.)
فهرسة مساهمة: Keywords: Endosomal escape; Gene delivery; Osmolysis; Polyethylenimine; Proton sponge
المشرفين على المادة: 0 (Drug Carriers)
0 (Polymers)
9002-98-6 (Polyethyleneimine)
9007-49-2 (DNA)
VCQ006KQ1E (Xylitol)
تواريخ الأحداث: Date Created: 20131105 Date Completed: 20141112 Latest Revision: 20220309
رمز التحديث: 20240513
DOI: 10.1016/j.nano.2013.10.005
PMID: 24184000
قاعدة البيانات: MEDLINE
الوصف
تدمد:1549-9642
DOI:10.1016/j.nano.2013.10.005