دورية أكاديمية
Quantitative dielectrophoretic tracking for characterization and separation of persistent subpopulations of Cryptosporidium parvum.
| العنوان: | Quantitative dielectrophoretic tracking for characterization and separation of persistent subpopulations of Cryptosporidium parvum. |
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| المؤلفون: | Su YH; Department of Electrical & Computer Engineering, University of Virginia, Charlottesville, VA 22904, USA. nswami@virginia.edu., Tsegaye M, Varhue W, Liao KT, Abebe LS, Smith JA, Guerrant RL, Swami NS |
| المصدر: | The Analyst [Analyst] 2014 Jan 07; Vol. 139 (1), pp. 66-73. Date of Electronic Publication: 2013 Nov 14. |
| نوع المنشور: | Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. |
| اللغة: | English |
| بيانات الدورية: | Publisher: Royal Society of Chemistry Country of Publication: England NLM ID: 0372652 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1364-5528 (Electronic) Linking ISSN: 00032654 NLM ISO Abbreviation: Analyst Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Cambridge : Royal Society of Chemistry Original Publication: London : Chemical Society |
| مواضيع طبية MeSH: | Cryptosporidium parvum/*chemistry , Cryptosporidium parvum/*isolation & purification , Electrophoresis/*methods , Oocysts/*chemistry, Animals ; Mice |
| مستخلص: | Microbial persistence to antibiotics is attributed to subpopulations with phenotypic variations that cause a spread of susceptibility levels, leading to the recurrence of infections and stability of biofilms. Herein, persistent oocyst subpopulations identified by animal infectivity and excystation assays during the disinfection of Cryptosporidium parvum, a water-borne pathogen capable of causing enteric infections at ultra-low doses, are separated and characterized by quantitative dielectrophoretic tracking over a wide frequency range (10 kHz-10 MHz). To enable the simultaneous and facile dielectrophoretic tracking of individual oocysts, insulator constrictions in a microfluidic channel are utilized to spatially modulate the localized field over the extent needed for defining oocyst trajectories and for obtaining high-resolution displacement versus time measurements under both, positive and negative dielectrophoresis. In this manner, by obviating the need for averaging dielectrophoretic data over a large collection region, the force response is more sensitive to differences in electrophysiology from sub-population fractions. Hence, the electrophysiology of sensitive and persistent oocysts after heat and silver nanoparticle treatments can be quantified by correlating the force response at low frequencies (<100 kHz) to the integrity of the oocyst wall and at high frequencies (0.4-1 MHz) to the sporozoites in the oocyst. This label-free method can characterize heterogeneous microbial samples with subpopulations of phenotypically different alterations, for quantifying the intensity of alteration and fraction with a particular alteration type. |
| معلومات مُعتمدة: | U54 AI057168 United States AI NIAID NIH HHS; U54 AI 057168 United States AI NIAID NIH HHS |
| تواريخ الأحداث: | Date Created: 20131115 Date Completed: 20140714 Latest Revision: 20211021 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1039/c3an01810e |
| PMID: | 24225592 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1364-5528 |
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| DOI: | 10.1039/c3an01810e |