دورية أكاديمية
Embryogenic and non-embryogenic cell lines of Daucus carota cloned from meristematic cell clusters: relation with cell ploidy determined by flow cytometry.
| العنوان: | Embryogenic and non-embryogenic cell lines of Daucus carota cloned from meristematic cell clusters: relation with cell ploidy determined by flow cytometry. |
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| المؤلفون: | Coutos-Thevenot P; Laboratoire de Physiologie Cellulaire Végétale, CNRS, F-91198, Gif sur Yvette, Cedex, France., Jouanneau JP, Brown S, Petiard V, Guern J |
| المصدر: | Plant cell reports [Plant Cell Rep] 1990 Mar; Vol. 8 (10), pp. 605-8. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Springer Country of Publication: Germany NLM ID: 9880970 Publication Model: Print Cited Medium: Print ISSN: 0721-7714 (Print) Linking ISSN: 07217714 NLM ISO Abbreviation: Plant Cell Rep Subsets: PubMed not MEDLINE |
| أسماء مطبوعة: | Original Publication: Berlin ; New York : Springer, 1981- |
| مستخلص: | The presence of totipotent and non-totipotent cells in embryogenic carrot cell suspension cultures was examined by cloning of cell microclusters. Forty clones were isolated and the distribution of their embryogenic potential was studied. Nonembryogenic, weakly and highly embryogenic cell lines were selected. After one year of subculture a second cloning round showed that the highly embryogenic and the non-embryogenic cell lines were homogenous and stable. A measurement of ploidy levels of clones by flow cytometry showed that the embryogenic clones were all diploid whereas the non-embryogenic were diploid or tetraploid. Hence, for our strain, there was a strict relationship between the tetraploid state and the inability to produce somatic embryos. |
| References: | Planta. 1980 Aug;149(3):292-7. (PMID: 24306302) Theor Appl Genet. 1982 Jun;61(2):113-6. (PMID: 24270331) Philos Trans R Soc Lond B Biol Sci. 1976 May 27;274(933):227-74. (PMID: 6977) Planta Med. 1984 Oct;50(5):427-31. (PMID: 17340345) Plant Physiol. 1985 Dec;79(4):988-91. (PMID: 16664558) Science. 1965 Feb 12;147(3659):756-8. (PMID: 17758199) |
| تواريخ الأحداث: | Date Created: 20131116 Date Completed: 20131118 Latest Revision: 20211021 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1007/BF00270064 |
| PMID: | 24232682 |
| قاعدة البيانات: | MEDLINE |
Find this article in full text from Springer Verlag. 
Full Text Finder | تدمد: | 0721-7714 |
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| DOI: | 10.1007/BF00270064 |